近年来,研究表明,一些细菌可能与结直肠癌(CRC)相关。在这项研究中,目的通过比较CRC患者和健康个体的结肠活检组织中这些细菌的数量,研究核梭杆菌和肠毒性脆弱拟杆菌(ETBF)在CRC病因中的作用.通过定量聚合酶链反应(qPCR)测定35例CRC和35例健康个体的结肠活检样品中F.nucleatum和ETBF的含量,并比较患者组和对照组的结果。与男性患者相比,女性CRC患者的组织中的F.nucleatum的检出率和量在统计学上显着较高(分别为p=0.003,p=0.013)。在ETBF的检出率和数量方面,女性和男性CRC患者的组织之间没有统计学上的显着差异(分别为p=0.521,p=0.515)。结果发现,在50-74岁的人群中,与对照组相比,女性和男性CRC患者的ETBF含量在统计学上显着高于对照组(分别为p=0.005,p=0.047),而与对照组相比,女性患者的F.nucleatum含量在统计学上显着高于对照组。然而,男性患者和对照组之间无差异(p=0.009,p=0.083).确定CRC患者组织中F.nucleatum的检出率和数量,不论年龄和性别,与对照组无统计学差异(分别为p=0.473,p=0.995),然而,发现ETBF的检出率和ETBF的量有统计学意义(分别为p=0.002,p=0.004)。已经确定ETBF可以在男性和女性的CRC的病因中发挥作用。核仁F.只适用于女性,在50-74岁的年龄范围内,进行常规CRC筛查时。
In recent years, it has been shown that some bacteria may be associated with colorectal cancer (CRC). In this study, it was aimed to investigate the role of Fusobacterium nucleatum and enterotoxigenic Bacteroides fragilis (ETBF) in the etiology of CRC by comparing the amounts of these bacteria in colon biopsy tissues of patients with CRC and healthy individuals. The amounts of F.nucleatum and ETBF were determined by quantitative polymerase chain reaction (qPCR) in colon biopsy samples taken from 35 CRC and 35 healthy individuals, and the results were compared in the patient and control groups. The detection rate and amounts of F.nucleatum were found to be statistically significantly higher in tissues of female patients with CRC compared to male patients (p= 0.003, p= 0.013, respectively). There was no statistically significant difference between the tissues of female and male patients with CRC in terms of detection rate and amount of ETBF (p= 0.521, p= 0.515, respectively). It was found that in the 50-74 age group, the amount of ETBF was statistically significantly higher in women and men with CRC compared to the controls (p= 0.005, p= 0.047, respectively), while the amount of F.nucleatum was statistically significantly higher in female patients compared to controls. However, no difference was found between male patients and controls (p= 0.009, p= 0.083). It was determined that the detection rate and amount of F.nucleatum in the tissues of patients with CRC, regardless of age and gender, were not statistically different from the controls (p= 0.473, p= 0.995, respectively), however, the detection rate of ETBF and the amount of ETBF were found to be statistically significantly higher (p= 0.002, p= 0.004, respectively). It has been determined that ETBF can play a role in the etiology of CRC in both men and women, and F.nucleatum only in women, in the age range of 50-74 years, when routine screenings for CRC are performed.