抗生素抗性基因(ARGs)是环境中新兴的污染物,已成为全球环境和健康关注的焦点。作为生物地球化学循环的重要参与者,红树林生态系统受到各种人为干扰,及其微生物群可能受到各种污染物如ARG的影响。本研究选取了海南红树林覆盖区的13个样带,中国用于沉积物样品采集。使用高通量定量聚合酶链反应(HT-qPCR)研究了ARGs和可移动遗传元件(MGEs)的丰度和多样性,高通量测序技术用于研究微生物的结构和多样性。在研究区域共检测到179种属于9种ARG类型的ARG,vanXD和vatE-01的检出率均为100%。ARGs的丰度为每克沉积物8.30×107-6.88×108个拷贝(每个16SrRNA基因1.27×10-2-3.39×10-2个拷贝),高于类似的研究,这些采样样带中ARGs的丰度存在差异。多药耐药基因(MRGs)所占比例最高(69.0%),这表明研究区域的ARGs污染非常复杂。ARG与MGE显著正相关,这表明ARGs的高水平与其自我增强有关。属水平的优势细菌是脱硫球菌,梭菌属,Rhodoplanes,芽孢杆菌,弧菌,肠球菌,细菌,假交替单胞菌,Paracocus,螺旋体,Mariprofundus,硫单胞菌,氨基杆菌,和Novoshingobium.133个细菌属与部分ARGs呈显著正相关。Chthonibacter,Flavisolibacter,Formivibrio,Kaistia,Moryella,MSBL3,Perlucidibaca,和周亚是海南红树林区沉积物中ARGs的主要潜在宿主,其中许多细菌是生物地球化学循环的重要参与者。研究结果有助于我们了解ARGs的分布和潜在寄主,为海南红树林生态系统的保护和管理提供科学依据。
Antibiotic resistance genes (ARGs) are emerging contaminants in the environment and have been highlighted as a worldwide environmental and health concern. As important participants in the biogeochemical cycles, mangrove ecosystems are subject to various anthropogenic disturbances, and its microbiota may be affected by various contaminants such as ARGs. This
study selected 13 transects of mangrove-covered areas in Hainan, China for sediment sample collection. The abundance and diversity of ARGs and mobile genetic elements (MGEs) were investigated using high-throughput quantitative polymerase chain reaction (HT-qPCR), and high-throughput sequencing was used to
study microbial structure and diversity. A total of 179 ARGs belonging to 9 ARG types were detected in the
study area, and the detection rates of vanXD and vatE-01 were 100%. The abundance of ARGs was 8.30 × 107-6.88 × 108 copies per g sediment (1.27 × 10-2-3.39 × 10-2 copies per 16S rRNA gene), which was higher than similar studies, and there were differences in the abundance of ARGs in these sampling transects. The multidrug resistance genes (MRGs) accounted for the highest proportion (69.0%), which indicates that the contamination of ARGs in the
study area was very complicated. The ARGs significantly positively correlated with MGEs, which showed that the high level of ARGs was related to its self-enhancement. The dominant bacteria at the genus level were Desulfococcus, Clostridium, Rhodoplanes, Bacillus, Vibrio, Enterococcus, Sedimentibacter, Pseudoalteromonas, Paracoccus, Oscillospira, Mariprofundus, Sulfurimonas, Aminobacterium, and Novosphingobium. There was a significant positive correlation between 133 bacterial genera and some ARGs. Chthoniobacter, Flavisolibacter, Formivibrio, Kaistia, Moryella, MSBL3, Perlucidibaca, and Zhouia were the main potential hosts of ARGs in the sediments of Hainan mangrove area, and many of these bacteria are important participants in biogeochemical cycles. The results contribute to our understanding of the distribution and potential hosts of ARGs and provide a scientific basis for the protection and management of Hainan mangrove ecosystem.