室内环境中真菌的存在与过敏和其他呼吸道症状有关。本研究的目的是使用测序和分子方法,包括下一代测序(NGS)方法,探索孟菲斯可见“发霉”(HVM)和不可见“非发霉”(HNM)的房屋(n=20)室内环境中的细菌和真菌群落及其丰度,TN,美国。从通风口和地面收集灰尘样本,并通过在IlluminaMiseq上扩增16SrRNA和ITS基因来分析细菌和真菌的总DNA。结果表明,Leptosphaerulina是HNM和HVM的通风口和地面样品中存在的最丰富的真菌属。同时,通风口和地面样品中最丰富的细菌属是丙酸杆菌和链球菌。气孔样品中的真菌群落多样性差异显著。室内灰尘样品中已知与呼吸道疾病相关的真菌种类的丰度相似,不管真菌在房子里的可见性。与呼吸道症状相关的真菌的存在与灰尘颗粒物(PM)等几个参数进行了比较,CO2水平,温度,和湿度。这些参数中的大多数与呼吸道疾病相关的真菌的存在呈正相关或负相关;然而,在p=0.05时,这些相关性均不显著。我们的结果表明,实施检测室内真菌的分子方法可能会加强常见的暴露和风险评估实践。
The presence of fungi in the indoor environment is associated with allergies and other respiratory symptoms. The aim of this study was to use sequencing and molecular methods, including next-generation sequencing (NGS) approaches, to explore the bacterial and fungal communities and their abundance in the indoor environment of houses (n = 20) with visible \"moldy\" (HVM) and nonvisible \"non-moldy\" (HNM) in Memphis, TN, USA. Dust samples were collected from air vents and ground surfaces, and the total DNA was analyzed for bacteria and fungi by amplifying 16S rRNA and ITS genes on the Illumina Miseq. Results indicated that Leptosphaerulina was the most abundant fungal genus present in the air vent and ground samples from HNM and HVM. At the same time, the most abundant bacterial genera in the air vent and ground samples were Propionibacterium and Streptococcus. The fungi community diversity was significantly different in the air vent samples. The abundance of fungal species known to be associated with respiratory diseases in indoor dust samples was similar, regardless of the visibility of fungi in the houses. The existence of fungi associated with respiratory symptoms was compared with several parameters like dust particulate matter (PM), CO2 level, temperature, and humidity. Most of these parameters are either positively or negatively correlated with the existence of fungi associated with respiratory diseases; however, none of these correlations were significant at p = 0.05. Our results indicate that implementing molecular methods for detecting indoor fungi may strengthen common exposure and risk assessment practices.