潜在精子捐献者的特征与这些潜在捐献者的接受率之间存在关系吗?
年龄≤35岁的潜在精子捐献者的接受率相对较高,结婚了,有了孩子,受过高等教育的人,春季和冬季的接受率也高于夏季和秋季。
由于高的排斥率和辍学率,招募捐献者到精子银行计划是困难和缓慢的。
从2006年至2019年,河南省人类精子银行共筛选了24040名潜在精子捐献者。
根据以下基线要求招募潜在的精子捐献者:身高168厘米或更高;年龄22-45岁;目前正在就读或高中毕业或以上。符合年龄标准的男人,高度,和教育水平被邀请进行精液质量筛查。精液样本的可接受标准包括液化时间<60min,体积≥2mL,精子浓度≥60×106/mL,渐进运动≥60%,解冻后运动≥40%,每瓶冷冻前活动精子总量>30×106/mL,每瓶解冻后活动精子总量>12×106/mL,冻融成活率≥60%。任何潜在的精子捐献者在两个连续的精液样本中符合可接受精液质量的最低标准,都被安排进行临床评估。体检,和实验室测试。分析精子供体排斥反应的原因。使用卡方检验比较接受和拒绝的供体的特征,并进行多因素logistic回归分析,以确定与潜在精子捐献者的接受率和性传播疾病(STDs)阳性率相关的因素.
仅23.38%(5620/24040)的潜在精子供体被接受。拒绝的前四个原因是精液质量欠佳(90.27%),性病(6.26%),退学(2.65%),和染色体异常(0.35%)。对STD供者排斥的最常见原因是支原体检测呈阳性(49.05%),其次是乙型肝炎病毒(27.56%),沙眼衣原体(4.68%),和大肠杆菌(3.03%)。在这项研究中,≤35岁男性的接受率明显高于>35岁男性(P<0.05)。受教育程度较高的男性的录取率也明显高于受教育程度较低的男性,已婚男人比未婚男人,且有儿童的男性高于无儿童的男性(P<0.05)。此外,春季和冬季的接受率明显高于夏季(P<0.05),而秋季的接受率并不明显高于夏季(P>0.05)。
这项研究不是为了分析生活习惯的影响,如饮酒和吸烟,潜在精子捐献者的接受率。
在这个匿名精子捐献者计划中,只有一小部分潜在的精子捐献者被接受。可能需要招募精子供体的新策略来提高接受率。在未来,我们可能必须针对年龄≤35岁且接受过高等教育的潜在精子捐献者,以提高接受率。
这项工作得到了河南省医学科技研究计划联合建设项目LHGJ20190389资助。作者报告没有利益冲突。
不适用。
Is there a relation between the characteristics of potential sperm donors and the acceptance rate of these potential donors?
A relatively higher acceptance rate was observed for potential sperm donors who were aged ≤ 35 years, were married, had children, and who had received higher education, and acceptance rates were also higher during spring and winter than summer and autumn.
Recruiting donors to a sperm bank program is difficult and slow owing to the high rates of rejection and dropout.
A total of 24040 potential sperm donors were screened by the Henan Human Sperm Bank from 2006 to 2019.
Potential sperm donors were recruited using the following baseline requirement: height of 168 cm or taller; age 22-45 years; currently attending or had graduated from high school or above. Men who met the criteria for age, height, and education level were invited for semen quality screening. The acceptable criteria for semen samples included liquefaction time < 60min, volume ≥ 2mL, sperm concentration ≥ 60 × 106/mL, progressive motility ≥ 60%, post-thaw motility ≥ 40%, pre-freezing total motile sperm per vial > 30 × 106/mL, post-thaw total motile sperm per vial > 12 × 106/mL, and freeze-thaw survival rate ≥ 60%. Any potential sperm donors meeting the minimum criteria for acceptable semen quality on two consecutive semen samples were scheduled for clinical assessment, physical examination, and laboratory tests. The reasons for sperm donor rejection were analyzed. The characteristics of accepted and rejected donors were compared using the chi-square test, and multivariate logistic regression analyses were conducted to identify factors associated with the acceptance rate of potential sperm donors and the positive rate of sexually transmitted diseases (STDs).
Only 23.38% (5620/24040) of potential sperm donors were accepted. The top four reasons for rejection were suboptimal semen quality (90.27%), STDs (6.26%), dropped out (2.65%), and chromosomal abnormalities (0.35%). The most common reason for the rejection of donors with an STD was a positive test for mycoplasmas (49.05%), followed by hepatitis B virus (27.56%), Chlamydia trachomatis (4.68%), and Escherichia coli (3.03%). n this
study, the acceptance rate for men aged ≤ 35 years was significantly higher than that for men aged >35 years (P < 0.05). The acceptance rates were also significantly higher for men with a higher education than for men with lower education, married men than unmarried men, and men with children than men without children (P < 0.05). Moreover, acceptance rates were significantly higher during spring and winter than during summer (P <0.05) but were not significantly higher during autumn than during summer (P >0.05).
This
study was not performed to analyze the effect of lifestyle habits, such as alcohol consumption and cigarette smoking, on the acceptance rate of potential sperm donors.
Only a small proportion of potential sperm donors were accepted in this anonymous sperm donor program. New strategies for sperm donor recruitment may be required to improve the acceptance rate. In the future, we may have to target potential sperm donors who are aged ≤ 35 years and who received higher education in order to improve the acceptance rate.
This work was supported by the Joint Construction Project of Henan Medical Science and Technology Research Plan under grant number LHGJ20190389. The authors report no conflict of interest.
N/A.