南非是许多土著和当地发展的绵羊的家园(NguniPedi,祖鲁,和NamaquaAfrikaner,Afrino,非洲人,Bezuidenhout非洲人,Damara,Dorper,DöhneMerino,肉大师,南非Merino,南非羊肉美利诺,VanRooy,和Dorper),山羊(SAveld,Tankwa,Imbuzi,班图人,布尔,和萨凡纳)和牛(Afrigus,Afrikaner,Bolowana,邦萨拉,Bovelder,Drakensberger,南非安格斯,南非瑞士乳业,南非Friesland,南非红,和VeldMaster)动物。这些品种需要较少的兽医服务,饲料,管理工作,为农村或贫困业主提供收入。然而,他们中的大多数都处于灭绝的危险之中,而有些则处于未知状态,需要立即进行保护干预。为了让濒危动物的基因更快地进步,重要的是要产生生产性动物,同时减少浪费,这可以通过按性别分类的精液来实现。因此,本系统综述旨在评估X和Y性别精液在反刍动物家畜中的前景,以及一些可用于解决性别分类较差的精液及其生育能力的解决方案.这项审查是通过收集和评估相关文章以及DAD-IS数据库中的数据纳入的。用于在线搜索文章的关键字是性别选择前,土著生态型,生育力,流式细胞术,人工授精,养护,改善精液性别。仔细查看所有文章后,PRISMA指南用于找到适合解决本评论目标的文章。性别分选精液是最近推出的一项技术,尤其是受到研究人员的关注,在保护计划中。基于性染色体(带有X和Y的染色体)的精液预选对于获得后代的所需性别并尽可能避免动物浪费至关重要。然而,多种因素会影响不同动物品种的精液质量,尤其是性别分类后。流式细胞术是用于选择男性和女性精子细胞并在此过程中丢弃死亡和异常精子细胞的常用方法。因此,然而,精子性别鉴定是一项很好的先进生殖技术(ART),它与氧化应激(OS)和DNA片段化(SDF)的产生有关。这些发现,因此,需要更多的创新研究来提出一种性别鉴定技术,该技术将在冷冻解冻的分选过程中保护精子细胞损伤。
South Africa is home to numerous indigenous and locally developed sheep (Nguni Pedi, Zulu, and Namaqua Afrikaner, Afrino, Africander, Bezuidenhout Africander, Damara, Dorper, Döhne Merino, Meat Master, South African Merino, South African Mutton Merino, Van Rooy, and Dorper), goat (SA veld, Tankwa, Imbuzi, Bantu, Boer, and Savanna) and cattle (Afrigus, Afrikaner, Bolowana, Bonsmara, Bovelder, Drakensberger, South African Angus, South African Dairy Swiss, South African Friesland, South African Red, and Veld Master) animals. These breeds require less veterinary service, feed, management efforts, provide income to rural and or poor owners. However, most of them are under extinction risks and some with unknown status hence, require immediate conservation intervention. To allow faster genetic progress on the endangered animals, it is important to generate productive animals while reducing wastages and this can be achieved through sex-sorted semen. Therefore, this systematic
review is aimed to evaluate the prospects of X and Y-sexed semen in ruminant livestock and some solutions that can be used to address poor sex-sorted semen and its fertility. This
review was incorporated through gathering and assessing relevant articles and through the data from the DAD-IS database. The keywords that were used to search articles online were pre-gender selection, indigenous ecotypes, fertility, flow cytometry, artificial insemination, conservation, and improving sexed semen. Following a careful
review of all articles, PRISMA guidelines were used to find the articles that are suitable to address the aim of this
review. Sex-sorted semen is a recently introduced technology gaining more attention from researchers particularly, in the conservation programs. Preselection of semen based on the sex chromosomes (X- and or Y-bearing chromosomes) is of paramount importance to obtain desired sex of the offspring and avoid animal wastage as much as possible. However, diverse factors can affect quality of semen of different animal species especially after sex-sorting. Flow cytometry is a common method used to select male and female sperm cells and discard dead and abnormal sperm cells during the process. Thus, sperm sexing is a good advanced reproductive technology (ART) however, it is associated with the production of oxidative stress (OS) and DNA fragmentation (SDF). These findings, therefore, necessitates more innovation studies to come up with a sexing technology that will protect sperm cell injuries during sorting in frozen-thawed.