■铜绿假单胞菌作为机会病原体产生几种毒力因子。这项研究评估了外切酶(exo)A的相对频率,U和S基因和整合子类(I,II,和III)在阿瓦兹烧伤患者的多药耐药临床铜绿假单胞菌中,伊朗西南部。
在该横断面研究中,从355个伤口样品中回收铜绿假单胞菌分离物。根据临床和实验室标准研究所,通过圆盘琼脂扩散法在Muller-Hinton琼脂上进行抗菌敏感性试验。如果MDR分离株同时对3种抗生素表现出耐药性,则将其定义。广泛耐药被定义为对除两个或更少的抗菌类别中的至少一种药物不敏感。一班的存在,II,和III整合子和毒力基因使用PCR测定法对提取的DNA进行测定。
■总的来说,145个临床铜绿假单胞菌分离物用生化和PCR试验证实。总的来说,35%(52/145)的分离株来自男性,64%(93/145)来自女性住院烧伤患者。铜绿假单胞菌对抗生素耐药率最高的是哌拉西林59%(n=86/145)和哌拉西林他唑巴坦57%(n=83/145)。总共100%的分离株对至少一种抗生素具有抗性。MDR和XDR铜绿假单胞菌的频率为60%和29%,分别。整合子I类的患病率,II,铜绿假单胞菌中的III为60%,7.58%,和3.44%,分别。IntI在MDR和XDR铜绿假单胞菌中更常见。此外,70(48%)的铜绿假单胞菌分离株没有整合子基因。此外,exoA,exoS,铜绿假单胞菌中的exoU频率为55%,55%,56%,分别。
■发现铜绿假单胞菌作为一种有效的病原体,在卫生界具有强毒力因子和高抗生素耐药性,可引起烧伤患者的难治性疾病。
UNASSIGNED: Pseudomonas aeruginosa as an opportunistic pathogen produces several virulence factors. This
study evaluated the relative frequency of exoenzymes (exo) A, U and S genes and integron classes (I, II, and III) among multi-drug-resistant clinical P. aeruginosa isolates from burn patients in Ahvaz, southwest of Iran.
UNASSIGNED: In this cross-sectional
study P. aeruginosa isolates were recovered from 355 wound samples. The antimicrobial susceptibility test was done by disk agar diffusion method on Muller-Hinton agar according to the Clinical and Laboratory Standards Institute. MDR isolates were defined if they showed simultaneous resistance to 3 antibiotics. Extensively drug-resistant was defined as nonsusceptibility to at least one agent in all but two or fewer antimicrobial categories. The presence of class I, II, and III integrons and virulence genes was determined using a PCR assay on extracted DNA.
UNASSIGNED: Overall, 145 clinical P. aeruginosa isolates were confirmed with biochemical and PCR tests. Overall, 35% (52/145) of the isolates were taken from males and 64% (93/145) from female hospitalized burn patients. The highest resistance rates of P. aeruginosa isolates to antibiotics were related to piperacillin 59% (n = 86/145) and piperacillin-tazobactam 57% (n = 83/145). A total of 100% of isolates were resistant to at least one antibiotic. MDR and XDR P. aeruginosa had a frequency of 60% and 29%, respectively. The prevalence of integron classes I, II, and III in P. aeruginosa was 60%, 7.58%, and 3.44%, respectively. IntI was more common in MDR and XDR P. aeruginosa isolates. In addition, 70(48%) of P. aeruginosa isolates did not harbor integron genes. Besides, exoA, exoS, and exoU in P. aeruginosa had a frequency of 55%, 55%, and 56%, respectively.
UNASSIGNED: It was found that P. aeruginosa as a potent pathogen with strong virulence factors and high antibiotic resistance in the health community can cause refractory diseases in burn patients.