背景:糖尿病伤口面临重大挑战,特别是在细菌感染和延迟愈合方面。因此,解决局部细菌问题和促进伤口加速愈合至关重要。在这次调查中,我们利用静电纺丝来制造包封MXene包封的微凝胶和壳聚糖/明胶聚合物的微凝胶/纳米纤维膜。
结果:薄膜敷料促进了近红外(NIR)下的程序化光热疗法(PPT)和轻度光热疗法(MPTT),展示快速和广泛的抗菌和生物膜破坏能力。PPT效果在52°C下在5分钟内实现快速灭菌,并在10分钟内分散成熟的生物膜。同时,通过调整NIR功率以引起局部温和加热(42°C),敷料刺激成纤维细胞增殖和迁移,显着增强血管化。此外,体内实验成功验证了薄膜敷料,强调其在解决糖尿病伤口的复杂性方面的巨大潜力。
结论:负载MXene微凝胶的纳米纤维敷料采用温度协调的光热疗法,有效地融合了高温灭菌和低温促进伤口愈合的优点。它表现得很快,广谱抗菌和生物膜破坏能力,特殊的生物相容性,对促进细胞增殖和血管化具有显著的作用。这些结果肯定了我们的纳米纤维敷料的功效,强调其在解决糖尿病伤口因感染而难以愈合的挑战方面的巨大潜力。
BACKGROUND: Diabetic wounds present significant challenges, specifically in terms of bacterial infection and delayed healing. Therefore, it is crucial to address local bacterial issues and promote accelerated wound healing. In this investigation, we utilized electrospinning to fabricate microgel/nanofiber membranes encapsulating MXene-encapsulated microgels and chitosan/gelatin polymers.
RESULTS: The film dressing facilitates programmed photothermal therapy (PPT) and mild photothermal therapy (MPTT) under near-infrared (NIR), showcasing swift and extensive antibacterial and biofilm-disrupting capabilities. The PPT effect achieves prompt sterilization within 5 min at 52 °C and disperses mature biofilm within 10 min. Concurrently, by adjusting the NIR power to induce local mild heating (42 °C), the dressing stimulates fibroblast proliferation and migration, significantly enhancing vascularization. Moreover, in vivo experimentation successfully validates the film dressing, underscoring its immense potential in addressing the intricacies of diabetic wounds.
CONCLUSIONS: The MXene microgel-loaded nanofiber dressing employs temperature-coordinated photothermal therapy, effectively amalgamating the advantageous features of high-temperature sterilization and low-temperature promotion of wound healing. It exhibits rapid, broad-spectrum antibacterial and biofilm-disrupting capabilities, exceptional biocompatibility, and noteworthy effects on promoting cell proliferation and vascularization. These results affirm the efficacy of our nanofiber dressing, highlighting its significant potential in addressing the challenge of diabetic wounds struggling to heal due to infection.