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Abstract：
Present scientific evidences about the biological activity and potential medical application of extracts derived from Marrubium friwaldskyanum Boiss. are limited. Therefore, our study was undertaken to define several main characteristics in this regard - in vitro cytotoxicity and antitumor properties, antibacterial activity and immunomodulatory potential. Extracts were obtained from different aerial parts of Marrubium friwaldskyanum - stems, leaves and flowers. The in vitro cytotoxicity and antitumor activity of the samples were evaluated by tetrazolium salt reduction tests and Neutral red uptake assays using four human cell lines (a normal fibroblastic and three adenocarcinoma cell lines/A549, HeLa, HT-29/) and by experiments with HT-29 tumor spheroids. Antibacterial activity toward Gram-negative (Escherichia coli) and Gram-positive (Bacillus cereus) species was assessed based on estimation of minimal inhibitory and minimal bactericidal concentrations as well as longitudinal studies on bacterial viability. Ex vivo assays with normal leukocytes were performed to define potential immunomodulatory activity of the extracts. Our results demonstrated selective antitumor activity of the extracts directed against colon adenocarcinoma HT-29 cells and cervical adenocarcinoma HeLa cell line. Metabolic activity of A549 lung adenocarcinoma cells was affected only by the sample derived from flowers. M. friwaldskyanum leaf and flower extracts showed the highest activity, which included reduction of HT-29 tumor spheroid growth and viability. The studied samples exhibited antibacterial activity against both bacterial species tested. Treatment with M. friwaldskyanum extracts affected specific leukocyte populations (HLA+, CD19+, CD11b+, CD25+ cells). These results demonstrate for the first time complex biological effects of extracts derived from M. friwaldskyanum and their potential to serve as a source of valuable compounds for the pharmaceutical industry.
摘要：
目前有关马鲁比草提取物的生物活性和潜在医学应用的科学证据。是有限的。因此,我们的研究是为了确定这方面的几个主要特征-体外细胞毒性和抗肿瘤特性，抗菌活性和免疫调节潜力。从Marrubiumfriwaldskyanum的不同地上部分获得提取物-茎，叶子和花朵。使用四种人细胞系（正常成纤维细胞和三种腺癌细胞系/A549，HeLa，HT-29/）和通过HT-29肿瘤球体的实验。基于最低抑制和最低杀菌浓度的估计以及对细菌活力的纵向研究，评估了对革兰氏阴性（大肠杆菌）和革兰氏阳性（蜡状芽孢杆菌）物种的抗菌活性。用正常白细胞进行离体测定以确定提取物的潜在免疫调节活性。我们的结果证明了提取物对结肠腺癌HT-29细胞和宫颈腺癌HeLa细胞系的选择性抗肿瘤活性。A549肺腺癌细胞的代谢活性仅受到来自花的样品的影响。M.friwaldskyanum叶和花提取物显示出最高的活性，其中包括HT-29肿瘤球体生长和生存力的降低。所研究的样品对所测试的两种细菌均表现出抗菌活性。用M.friwaldskyanum提取物治疗会影响特定的白细胞群体(HLA+，CD19+,CD11b+,CD25+细胞）。这些结果首次证明了源自M.friwaldskyanum的提取物的复杂生物学效应及其作为制药工业有价值化合物来源的潜力。
