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Abstract：
Chitosan, a biopolymer obtained from chitin, is known for its remarkable adsorption abilities for dyes, drugs, and fats, and its diverse array of antibacterial characteristics. This study explores the extraction and characterization of chitosan from the mycelium of Amanita phalloides. The moisture content, ash content, water binding capacity, fat binding capacity, and degree of deacetylation of the extracted chitosan were determined. The chitosan exhibited a high yield of 70%, crystallinity of 49.07%, a degree of deacetylation of 86%, and potent antimicrobial properties against both Gram-negative and Gram-positive bacteria. The study also examined the adsorption capabilities of chitosan to remove methylene blue (MB) dye by analysing specific factors like pH, reaction time, and MB concentration using the response surface model. The highest degree of MB dye removal was 91.6% at a pH of 6, a reaction time of around 60 min and an initial dye concentration of 16 ppm. This experimental design can be applied for chitosan adsorption of other organic compounds such as dyes, proteins, drugs, and fats.
摘要：
壳聚糖,从几丁质中获得的生物聚合物，以其对染料的卓越吸附能力而闻名，毒品,和脂肪，以及其多样化的抗菌特性。本研究探索了从鸡毒菌丝体中提取壳聚糖并进行表征。水分含量，灰分含量,水结合能力，脂肪结合能力，并测定了提取的壳聚糖的脱乙酰度。壳聚糖具有70%的高收率,结晶度为49.07%，86％的脱乙酰度，和有效的抗革兰氏阴性和革兰氏阳性细菌的抗菌性能。该研究还通过分析pH等特定因素来检查壳聚糖去除亚甲基蓝（MB）染料的吸附能力，反应时间,和MB浓度使用响应面模型。在pH为6，反应时间约为60分钟和初始染料浓度为16ppm时，MB染料的最高去除率为91.6％。本实验设计可应用于壳聚糖对染料等其他有机化合物的吸附,蛋白质，毒品,和脂肪。
