The partial denitrification (PD) coupled with anaerobic ammonium oxidation (Anammox) (PD/A) process is a unique biological denitrification method for sewage that concurrently removes nitrate (NO3--N) and ammonium (NH4+-N) in sewage. Comparing PD/A to conventional nitrification and denitrification technologies, noticeable improvements are shown in energy consumption, carbon source demand, sludge generation and emissions of greenhouse gasses. The PD is vital to obtaining nitrites (NO2--N) in the Anammox process. This paper provided valuable insight by introduced the basic principles and characteristics of the process and then summarized the strengthening strategies. The functional microorganisms and microbial competition have been discussed in details, the S-dependent denitrification-anammox has been analyzed in this review paper. Important factors affecting the PD/A process were examined from different aspects, and finally, the paper pointed out the shortcomings of the coupling process in experimental research and engineering applications. Thus, this research provided insightful information for the PD/A process\'s optimization technique in later treating many types of real and nitrate-based wastewater. The review paper also provided the prospective economic and environmental position for the actual design implementation of the PD/A process in the years to come.

Anaerobic ammonium oxidation has been considered as an environmental-friendly and energy-efficient biological nitrogen removal (BNR) technology. Recently, new reaction pathway for ammonium oxidation under anaerobic condition had been discovered. In addition to nitrite, iron trivalent, sulfate, manganese and electrons from electrode might be potential electron acceptors for ammonium oxidation, which can be coupled to traditional BNR process for wastewater treatment. In this paper, the pathway and mechanism for ammonium oxidation with various electron acceptors under anaerobic condition is studied comprehensively, and the research progress of potentially functional microbes is summarized. The potential application of various electron acceptors for ammonium oxidation in wastewater is addressed, and the N2O emission during nitrogen removal is also discussed, which was important greenhouse gas for global climate change. The problems remained unclear for ammonium oxidation by multi-electron acceptors and potential interactions are also discussed in this review.

Anaerobic ammonium oxidation (anammox) is a promising biological method for treating nitrogen-rich, low-carbon wastewater. However, the application of anammox technology in actual engineering is easily limited by environmental factors. Considerable progress has been investigated in recent years in anammox restoration strategies, significantly addressing the challenge of poor reaction performance following inhibition. This review systematically outlines the strategies employed to recover anammox performance following inhibition by conventional environmental factors and emerging pollutants. Additionally, comprehensive summaries of strategies aimed at promoting anammox activity and enhancing nitrogen removal performance provide valuable insights into the current research landscape in this field. The review contributes to a comprehensive understanding of restoration strategies of anammox-based technologies.

Nowadays, the discharge of wastewater is a global concern due to the damage caused to human and environmental health. Wastewater treatment has progressed to provide environmentally and economically sustainable technologies. The biological treatment of wastewater is one of the fundamental bases of this field, and the employment of new technologies based on granular biofilm systems is demonstrating success in tackling the environmental issues derived from the discharge of wastewater. The granular-conforming microorganisms must be evaluated as functional entities because their activities and functions for removing pollutants are interconnected with the surrounding microbiota. The deep knowledge of microbial communities allows for the improvement in system operation, as the proliferation of microorganisms in charge of metabolic roles could be modified by adjustments to operational conditions. This is why engineering must consider the intrinsic microbiological aspects of biological wastewater treatment systems to obtain the most effective performance. This review provides an extensive view of the microbial ecology of biological wastewater treatment technologies based on granular biofilms for mitigating water pollution.

Treatment of high-strength wastewater is critical for the aquatic environment and receiving water bodies around the globe. Untreated or partially treated high-strength wastewater may cause severe damage to the existing water bodies. Various high-rate anaerobic bioreactors have been developed in the last decades for treating high-strength wastewater. High-rate anaerobic bioreactors are effective in treating industrial wastewater and provide energy in the form of methane as well. However, the physical or chemical properties of high-strength industrial wastewater, sometimes, disrupt the functioning of a high-rate anaerobic bioreactor. For example, the disintegration of granular sludge in up flow anaerobic sludge blanket reactor or membrane blocking in an anaerobic membrane bioreactor are the results of a high-strength wastewater treatment which hamper the proper functioning and may harm the wastewater treatment plant economically. Biochar, if added to these bioreactors, may help to alleviate the ill-functioning of high-rate anaerobic bioreactors. The primary mechanisms by biochar work in these bioreactors are direct interspecies electron transfer, microbial immobilization, or gene level alternations in microbial structure. The present article explores and reviews the recent application of biochar in a high-rate anaerobic bioreactor treating high-strength industrial wastewater.

With the rise of the concept of carbon neutrality, the current wastewater treatment process of industrial organic wastewater is moving towards the goal of energy conservation and carbon emission reduction. The advantages of anaerobic digestion (AD) processes in industrial organic wastewater treatment for bio-energy recovery, which is in line with the concept of carbon neutrality. This study summarized the significance and advantages of the state-of-the-art AD processes were reviewed in detail. The application of expanded granular sludge bed (EGSB) reactors and anaerobic membrane bioreactor (AnMBR) were particularly introduced for the effective treatment of industrial organic wastewater treatment due to its remarkable prospect of engineering application for the high-strength wastewater. This study also looks forward to the optimization of the AD processes through the enhancement strategies of micro-aeration pretreatment, acidic-alkaline pretreatment, co-digestion, and biochar addition to improve the stability of the AD system and energy recovery from of industrial organic wastewater. The integration of anaerobic ammonia oxidation (Anammox) with the AD processes for the post-treatment of nitrogenous pollutants for the industrial organic wastewater is also introduced as a feasible carbon-neutral process. The combination of AnMBR and Anammox is highly recommended as a promising carbon-neutral process for the removal of both organic and inorganic pollutants from the industrial organic wastewater for future perspective. It is also suggested that the AD processes combined with biological hydrogen production, microalgae culture, bioelectrochemical technology and other bio-processes are suitable for the low-carbon treatment of industrial organic wastewater with the concept of carbon neutrality in future.

The proliferation of novel wastewater treatment processes has marked recent years, becoming particularly pertinent in light of the strive for carbon neutrality. One area of growing attention within this context is nitrous oxide (N2O) production and emission. This review provides a comprehensive overview of recent research progress on N2O emissions associated with novel wastewater treatment processes, including Anammox, Partial Nitrification, Partial Denitrification, Comammox, Denitrifying Phosphorus Removal, Sulfur-driven Autotrophic Denitrification and n-DAMO. The advantages and challenges of these processes are thoroughly examined, and various mitigation strategies are proposed. An interesting angle that delve into is the potential of endogenous denitrification to act as an N2O sink. Furthermore, the review discusses the potential applications and rationale for novel Anammox-based processes to reduce N2O emissions. The aim is to inform future technology research in this area. Overall, this review aims to shed light on these emerging technologies while encouraging further research and development.

The anaerobic ammonium oxidation (anammox) process has been developed as one of the optimal alternatives to the conventional biological nitrogen removal process because of its high nitrogen removal capacity and low energy consumption. However, the slow growth rate of anammox bacteria and its high sensitivity to environmental changes have resulted in fewer anammox sludge sources for process start-up and a lengthy start-up period. Given that anammox microorganisms tend to aggregate, granular-anammox sludge is a frequent byproduct of the anammox process. In this study, we review state-of-the-art strategies for promoting the formation of anammox granules and the start-up of the anammox process based on the literature of the past decade. These strategies are categorized as the transformation of alternative sludge, the addition of accelerators, the introduction of functional carriers, and the implementation of other physical methods. In addition, the formation mechanism of anammox granules, the operational performance of various strategies, and their promotion mechanisms are introduced. Finally, prospects are presented to indicate the gaps in contemporary research and the potential future research directions. This review functions as a summary guideline and theoretical reference for the cultivation of granular-anammox sludge, the start-up of the anammox process, and its practical application.

Full-scale anaerobic ammonium oxidation (anammox) engineering applications are vastly limited by the sensitivity of anammox bacteria to the complex mainstream ambience factors. Therefore, it is of great necessity to comprehensively summarize and overcome performance-related challenges in mainstream anammox process at the macro/micro level, including the macroscopic process variable regulation and microscopic biological metabolic enhancement. This article systematically reviewed the recent important advances in the enrichment and retention of anammox bacteria and main factors affecting metabolic regulation under mainstream conditions, and proposed key strategies for the related performance optimization. The characteristics and behavior mechanism of anammox consortia in response to mainstream environment were then discussed in details, and we revealed that the synergistic nitrogen metabolism of multi-functional bacterial genera based on anammox microbiome was conducive to mainstream anammox nitrogen removal processes. Finally, the critical outcomes of anammox extracellular electron transfer (EET) at the micro level were well presented, carbon-based conductive materials or exogenous electron shuttles can stimulate and mediate anammox EET in mainstream environments to optimize system performance from a micro perspective. Overall, this review advances the extensive implementation of mainstream anammox practice in future as well as shedding new light on the related EET and microbial mechanisms.

Sustainable development goal 6 emphasizes the efficient management of water resources, wastewater treatment, and reuse of treated wastewater. Removing nitrogen from wastewater was an economically expensive and energy-consuming wastewater treatment process. The discovery of anammox changes the paradigm of wastewater treatment. Nevertheless, coupling anammox with partial nitrification (PN-anammox) has been an immensely rewarding and scientifically supported side-stream wastewater treatment process. However, the PN-anammox process is inherited with severe issues of higher effluent nitrate and lower nitrogen removal efficiency at a lower temperature. Thus, it is evident that PN-anammox cannot meet the desired target without the involvement of others nitrogen cycle bacteria. The nitrate reduction pathways such as denitrifying anaerobic methane-oxidizing (DAMO) microbes, partial denitrification (PD), and dissimilatory nitrate reduction to ammonium (DNRA) seem the best alternative to reduce nitrate into nitrite or ammonium for anammox supplement. From the perspective of the environment, the coupling of anammox with PD, DAMO, and DNRA alleviates the use of organic matter, decreases greenhouse gas release, and reduces energy consumption. This review comprehensively discussed the importance and application perspective of anammox with diverse nitrate-reducing bacteria. Furthermore, research is still needed about DAMO-anammox and DNRA-anammox to achieve higher nitrogen removal efficiency. Future research should incorporate emerging pollutants removal in the anammox coupling process. This review will provide deep insight into the design of energy-efficient and carbon-neutral nitrogen removal from wastewater.