目的:评估胶原支架(CS)的体外吸收行为,表面结构,溶胀行为,和生理模拟环境中的机械性能,与猪天然结缔组织相比。
方法:三种测试材料-一种猪胶原蛋白基质(p-CM),两种无细胞真皮基质(猪=p-ADM,使用高精度量表检查了同种异体=a-ADM)-和作为对照材料的猪天然结缔组织(p-CTG)在四种溶液中的吸收。溶液是人工唾液(AS)和模拟体液(SBF),有和没有胶原酶(0.5U/ml,37℃)。此外,在暴露于AS或SBF之前和之后,使用扫描电子显微镜(SEM)分析CS的表面结构。通过测量磷酸盐缓冲盐水(PBS)中的体积变化和液体吸收能力来评价CS的溶胀行为。最后,在PBS中使用循环压缩测试研究CS和p-CTG的机械性能。
结果:含有胶原酶的溶液在4小时后显示出高的再吸收率,测试材料之间存在显着差异(p<0.04),8h和24h,24小时后范围为54.1至100%。SEM图像显示所有未处理的标本中交联的胶原蛋白结构。与ADM不同,p-CM和p-ADM支架呈片状结构。在测试材料中,溶胀率和单位面积的流体吸收能力在13.4至25.5%的范围内,并显示以下模式:p-CM>a-ADM>p-ADM。P-CM表现出比P-ADM更高的弹性性能,而A-ADM,像p-CTG,在增加的压力下几乎不可压缩,失去了结构完整性。
结论:胶原支架的物理性质差异很大,如吸收和溶胀行为和弹性特性,取决于它们的微观结构和组成。临床应用时,应该考虑这些差异,以实现预期的结果。
To evaluate collagen scaffolds (CS) in terms of their in vitro resorption behavior, surface structure, swelling behavior, and mechanical properties in physiologically simulated environments, compared with porcine native connective tissue.
Three test materials-one porcine collagen matrix (p-CM), two acellular dermal matrices (porcine = p-ADM, allogenic = a-ADM)-and porcine native connective tissue (p-CTG) as a control material were examined for resorption in four solutions using a high-precision scale. The solutions were artificial saliva (AS) and simulated body fluid (SBF), both with and without collagenase (0.5 U/ml at 37 °C). In addition, the surface structures of CS were analyzed using a scanning electron microscope (SEM) before and after exposure to AS or SBF. The swelling behavior of CS was evaluated by measuring volume change and liquid absorption capacity in phosphate-buffered saline (PBS). Finally, the mechanical properties of CS and p-CTG were investigated using cyclic compression testing in PBS.
Solutions containing collagenase demonstrated high resorption rates with significant differences (p < 0.04) between the tested materials after 4 h, 8 h and 24 h, ranging from 54.1 to 100% after 24 h. SEM images revealed cross-linked collagen structures in all untreated specimens. Unlike a-ADM, the scaffolds of p-CM and p-ADM displayed a flake-like structure. The swelling ratio and fluid absorption capacity per area ranged from 13.4 to 25.5% among the test materials and showed following pattern: p-CM > a-ADM > p-ADM. P-CM exhibited higher elastic properties than p-ADM, whereas a-ADM, like p-CTG, were barely compressible and lost structural integrity under increasing pressure.
Collagen scaffolds vary significantly in their physical properties, such as resorption and swelling behavior and elastic properties, depending on their microstructure and composition. When clinically applied, these differences should be taken into consideration to achieve the desired outcomes.