PDF(Pubmed)
Abstract：
CONCLUSIONS: Our data link the miR165/166- and miR160-mediated regulatory modules to ROS and seed formation. Trade-offs of seed size, weight, and number probably require control of the expression of miR165/166 by miR160, modulation of ROS metabolism by miR165/166, and miR160 abundance by ROS-induced oxidative modifications The cycle of plant life and its yield productivity depends fundamentally on the establishment of the trade-offs of seed size, weight, and number. For annual plants, seed number should simply be a positive function of vegetative biomass and a negative function of seed size and/or weight. However, extensive natural variation within species is observed for these traits, for which an optimal solution is environmentally dependent. Understanding the miRNA-mediated post-transcriptional regulation of gene expression determining seed phenotype and number is crucial from both an evolutionary and applied perspective. Although extensive research has concentrated on the individual roles of miRNAs in plant life, fewer studies have centred on their functional interactions, hence this study aimed to examine whether the module of miR165/miR166 and/or miR160 interactions is involved in forming Arabidopsis thaliana seeds, and/or has an impact on their features. Considering that reactive oxygen species (ROS) are among key players in seed-related processes, it was also intriguing to verify if the mechanism of action of these miRNAs is associated with the ROS pathway. The plant material used in this study consisted of flower buds, green siliques, and freshly harvested seeds, of wild type (WT), and STTM165/166 and STTM160 × 165/166 mutants of A. thaliana plants which are powerful tools for functional analysis of miRNAs in plants. The novel results obtained during physiological phenotyping together with two-tailed qRT-PCR analysis of mature miR165, miR166, miR160, and spectrofluorimetric measurement of apoplastic hydrogen peroxide (H2O2) for the first time revealed that interaction between miR165/miR166 and miR160 may regulate seed size, weight and number in ROS-dependent manner.
摘要：
结论：我们的数据将miR165/166和miR160介导的调节模块与ROS和种子形成联系起来。种子大小的权衡，体重,和数量可能需要通过miR160控制miR165/166的表达，通过miR165/166调节ROS代谢，以及通过ROS诱导的氧化修饰来控制miR160的丰度。植物生命的周期及其产量生产力从根本上取决于种子大小权衡的建立，体重,和数量。对于一年生植物，种子数量应该只是营养生物量的正函数和种子大小和/或重量的负函数。然而,这些性状在物种内观察到广泛的自然变异，其中一个最佳的解决方案是依赖于环境的。从进化和应用的角度来看，了解miRNA介导的决定种子表型和数量的基因表达的转录后调控至关重要。尽管广泛的研究集中在miRNAs在植物生命中的个体作用上，很少有研究集中在它们的功能相互作用上，因此，本研究旨在检查miR165/miR166和/或miR160相互作用的模块是否参与形成拟南芥种子，和/或对其特征有影响。考虑到活性氧（ROS）是种子相关过程中的关键参与者，验证这些miRNAs的作用机制是否与ROS途径相关也很有趣。本研究中使用的植物材料由花蕾组成，绿色角果，和新鲜收获的种子，野生型（WT），和STTM165/166和STTM160×165/166突变体是植物中miRNA功能分析的有力工具。在生理表型分析以及成熟miR165，miR166，miR160的双尾qRT-PCR分析以及脱体过氧化氢（H2O2）的荧光光谱测量过程中获得的新结果首次表明，miR165/miR166和miR160之间的相互作用可能会调节种子大小，ROS依赖方式的重量和数量。
