背景:睾丸是雄性哺乳动物的重要生殖器官,是精子发生的场所,雄激素合成,和分泌。非编码RNA(ncRNAs)在各种生物过程中起着重要的调节作用。然而,ncRNAs在牦牛睾丸发育和精子发生过程中的调控作用尚不清楚。
结果:在这项研究中,我们比较了环状RNA(circularRNAs)的表达谱,microRNAs(miRNAs),和信使RNA(mRNA)在6个月时收集的牦牛睾丸组织样本(Y6M),18个月(Y18M),4年(Y4Y)。使用RNA测序(RNA-Seq),我们观察到在不同睾丸发育阶段收集的样本中ncRNAs的表达模式存在显著差异。22个差异表达(DE)circRNAs,69DEmiRNA,在Y6M中检测到64个DEmRNA,Y18M,和Y4Y睾丸样本,分别。基因本体论(GO)和京都基因和基因组百科全书(KEGG)分析的结果表明,DEmiRNA的预测靶基因,和DEmRNA与与精子合成相关的信号通路和GO术语特异性相关,精子活力,睾丸发育,如细胞周期,Wnt信号通路,MAPK信号通路,GnRH信号通路,和精子发生。对circRNA-miRNA-mRNA网络的分析表明,一些DEncRNAs,包括miR-574,miR-449a,CDC42和CYP11A1等,可能与睾丸精子发生有关。同时,观察到各种circRNA-miRNA相互作用对。
结论:我们的发现提供了一个circRNAs数据库,miRNA,和mRNAs在不同发育阶段牦牛睾丸组织中的表达谱,并详细了解牦牛睾丸发育中ncRNAs的调控网络,提供有助于阐明牦牛睾丸发育的分子机制的数据。
BACKGROUND: The Testis is an important reproductive organ in male mammals and the site for spermatogenesis, androgen synthesis, and secretion. Non-coding RNAs (ncRNAs) play an important regulatory role in various biological processes. However, the regulatory role of ncRNAs in the development of yak testes and spermatogenesis remains largely unclear.
RESULTS: In this study, we compared the expression profiles of circular RNAs (circRNAs), microRNAs (
miRNAs), and messenger RNAs (mRNAs) in yak testicular tissue samples collected at 6 months (Y6M), 18 months (Y18M), and 4 years (Y4Y). Using RNA sequencing (RNA-Seq), we observed a significant difference in the expression patterns of ncRNAs in the samples collected at different testicular development stages. Twenty-two differentially expressed (DE) circRNAs, 69 DE
miRNAs, and 64 DE mRNAs were detected in Y6M, Y18M, and Y4Y testicular samples, respectively. The results of gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses showed that the source genes of DE circRNAs, predicted target genes of DE
miRNAs, and DE mRNAs were specifically associated with signaling pathways and GO terms that were related to sperm synthesis, sperm vitality, and testicular development, such as cell cycle, Wnt signaling pathway, MAPK signaling pathway, GnRH signaling pathway, and spermatogenesis. The analysis of the circRNA-miRNA-mRNA network revealed that some DE ncRNAs, including miR-574, miR-449a, CDC42, and CYP11A1, among others, may be involved in testicular spermatogenesis. Concurrently, various circRNA-miRNA interaction pairs were observed.
CONCLUSIONS: Our findings provide a database of circRNAs,
miRNAs, and mRNAs expression profiles in testicular tissue of yaks at different developmental stages and a detailed understanding of the regulatory network of ncRNAs in yak testicular development and provide data that can help elucidate the molecular mechanisms underlying yak testicular development.