PDF(Pubmed)
Abstract：
This study aimed to evaluate the influence of combined intermittent fasting (IF) and high-intensity interval training (HIIT) on morphology, caspase-independent apoptosis signaling pathway, and myostatin expression in soleus and gastrocnemius (white portion) muscles from healthy rats. Sixty-day-old male Wistar rats (n = 60) were divided into four groups: control (C), IF, high-intensity-interval training (T), and high-intensity-interval training and intermittent fasting (T-IF). The C and T groups received ad libitum chow daily; IF and T-IF received the same standard chow every other day. Animals from T and T-IF underwent a HIIT protocol five times a week for 12 weeks. IF reduced gastrocnemius mass and increased pro-apoptotic proteins apoptosis-inducing factor (AIF) and endonuclease G (EndoG) in soleus and cleaved-to-non-cleaved PARP-1 ratio and myostatin expression in gastrocnemius white portion. HIIT increased AIF and apoptosis repressor with caspase recruitment domain expression in soleus and cleaved-to-total PARP-1 ratio in gastrocnemius muscle white portion. The combination of IF and HIIT reduced fiber cross-sectional area in both muscles, increased EndoG and AIF expression, and decreased cleaved-to-non-cleaved PARP-1 ratio in gastrocnemius muscle white portion. Muscle responses to IF and HIIT are directly impacted by the muscle fiber type composition and are modulated, at least in part, by myostatin and caspase-independent apoptosis signaling.
摘要：
本研究旨在评估间歇性禁食（IF）和高强度间歇训练（HIIT）对形态学的影响。caspase非依赖性凋亡信号通路,和肌肉生长抑制素在健康大鼠比目鱼肌和腓肠肌(白色部分)中的表达。将60日龄雄性Wistar大鼠（n=60）分为四组：对照组（C），如果,高强度间歇训练(T)，高强度间歇训练和间歇性禁食（T-IF）。C和T组每天随意接受食物；IF和T-IF每隔一天接受相同的标准食物。来自T和T-IF的动物经历HIIT方案,每周五次,持续12周。IF减少腓肠肌质量，增加比目鱼肌中的促凋亡蛋白凋亡诱导因子（AIF）和核酸内切酶G（EndoG）以及腓肠肌白色部分中切割与未切割PARP-1的比例和肌肉生长抑制素的表达。HIIT增加了AIF和凋亡阻遏物，比目鱼肌中的caspase募集结构域表达和腓肠肌白色部分的PARP-1裂解与总比例。IF和HIIT的组合减少了两个肌肉的纤维横截面积，EndoG和AIF表达增加，腓肠肌白色部分的切割与未切割的PARP-1比率降低。对IF和HIIT的肌肉反应直接受到肌纤维类型组成的影响，并受到调节，至少在某种程度上,肌肉生长抑制素和caspase非依赖性凋亡信号。
