Abstract：
The Caenorhabditis elegans germline is arranged spatiotemporally and is therefore a powerful model system for the interrogation of meiotic molecular dynamics. Coupling this property with the temporal control that the auxin-inducible degron (AID) system allows can unveil new/unappreciated roles for critical meiotic factors in specific germline regions. Here we describe a widely used approach for the introduction of degron tags to specific targets and provide a procedure for applying the AID system to C. elegans meiotic DSB repair dynamics in the germline.
摘要：
秀丽隐杆线虫种系时空排列，因此是用于询问减数分裂分子动力学的强大模型系统。将此特性与生长素诱导型degron（AID）系统允许的时间控制相结合，可以揭示特定种系区域中关键减数分裂因子的新/未被重视的作用。在这里，我们描述了一种广泛使用的将degron标签引入特定靶标的方法，并提供了将AID系统应用于线虫减数分裂DSB修复动力学的程序。
