{Reference Type}: Journal Article
{Title}: Temporal Analysis of DSB Repair Outcome in Caenorhabditis elegans Meiosis.
{Author}: Hicks T;Silva N;Smolikove S;
{Journal}: Methods Mol Biol
{Volume}: 2818
{Issue}: 0
{Year}: 2024
暂无{DOI}: 10.1007/978-1-0716-3906-1_13
{Abstract}: The Caenorhabditis elegans germline is arranged spatiotemporally and is therefore a powerful model system for the interrogation of meiotic molecular dynamics. Coupling this property with the temporal control that the auxin-inducible degron (AID) system allows can unveil new/unappreciated roles for critical meiotic factors in specific germline regions. Here we describe a widely used approach for the introduction of degron tags to specific targets and provide a procedure for applying the AID system to C. elegans meiotic DSB repair dynamics in the germline.