%0 Journal Article
%T Temporal Analysis of DSB Repair Outcome in Caenorhabditis elegans Meiosis.
%A Hicks T
%A Silva N
%A Smolikove S
%J Methods Mol Biol
%V 2818
%N 0
%D 2024
%M 39126476
暂无%R 10.1007/978-1-0716-3906-1_13
%X The Caenorhabditis elegans germline is arranged spatiotemporally and is therefore a powerful model system for the interrogation of meiotic molecular dynamics. Coupling this property with the temporal control that the auxin-inducible degron (AID) system allows can unveil new/unappreciated roles for critical meiotic factors in specific germline regions. Here we describe a widely used approach for the introduction of degron tags to specific targets and provide a procedure for applying the AID system to C. elegans meiotic DSB repair dynamics in the germline.