Abstract：
During meiosis, Spo11 generates DNA double-strand breaks to induce recombination, becoming covalently attached to the 5\' ends on both sides of the break during this process. Such Spo11 \"covalent complexes\" are transient in wild-type cells, but accumulate in nuclease mutants unable to initiate repair. The CC-seq method presented here details how to map the location of these Spo11 complexes genome-wide with strand-specific nucleotide-resolution accuracy in synchronized Saccharomyces cerevisiae meiotic cells.
摘要：
在减数分裂期间,spo11产生DNA双链断裂诱导重组，在此过程中，共价连接到断裂两侧的5'末端。这种“共价复合物”在野生型细胞中是瞬时的，但积累的核酸酶突变体无法启动修复。此处提供的CC-seq方法详细介绍了如何在同步酿酒酵母减数分裂细胞中以链特异性核苷酸分辨率精度在全基因组范围内绘制这些Po11复合物的位置。
