%0 Journal Article
%T CC-seq: Nucleotide-Resolution Mapping of Spo11 DNA Double-Strand Breaks in S. cerevisiae Cells.
%A Brown GGB
%A Gittens WH
%A Allison RM
%A Oliver AW
%A Neale MJ
%J Methods Mol Biol
%V 2818
%N 0
%D 2024
%M 39126464
暂无%R 10.1007/978-1-0716-3906-1_1
%X During meiosis, Spo11 generates DNA double-strand breaks to induce recombination, becoming covalently attached to the 5' ends on both sides of the break during this process. Such Spo11 "covalent complexes" are transient in wild-type cells, but accumulate in nuclease mutants unable to initiate repair. The CC-seq method presented here details how to map the location of these Spo11 complexes genome-wide with strand-specific nucleotide-resolution accuracy in synchronized Saccharomyces cerevisiae meiotic cells.