{Reference Type}: Journal Article
{Title}: CC-seq: Nucleotide-Resolution Mapping of Spo11 DNA Double-Strand Breaks in S. cerevisiae Cells.
{Author}: Brown GGB;Gittens WH;Allison RM;Oliver AW;Neale MJ;
{Journal}: Methods Mol Biol
{Volume}: 2818
{Issue}: 0
{Year}: 2024
暂无{DOI}: 10.1007/978-1-0716-3906-1_1
{Abstract}: During meiosis, Spo11 generates DNA double-strand breaks to induce recombination, becoming covalently attached to the 5' ends on both sides of the break during this process. Such Spo11 "covalent complexes" are transient in wild-type cells, but accumulate in nuclease mutants unable to initiate repair. The CC-seq method presented here details how to map the location of these Spo11 complexes genome-wide with strand-specific nucleotide-resolution accuracy in synchronized Saccharomyces cerevisiae meiotic cells.