PDF(Pubmed)
Abstract：
The production of date syrup yields a substantial amount of date press cake (DPC), fibrous and moisturising material with great potential for generating value through bioprocessing. However, the recalcitrant structure of DPC affects the yield of products in bioprocesses. To boost the accessibility of the structure as well as increase the soluble fraction of carbohydrates and facilitate further enzymatic hydrolysis, hydrothermal and dilute acid (0.5% (v/v) sulfuric acid) pretreatments as cost-effective and feasible methods were applied on DPC at relatively low temperatures (80, 100, 120 and 140 °C) and reaction times (60 and 90 min). The success in pretreatment was then evaluated by a post-enzymatic treatment using an enzyme cocktail of cellulases and hemicelluloses. Based on total accessible sugar with minimum produced inhibitors, an optimal operating condition was considered acid pretreatment at 120 °C for 90 min with a 55.02% increase in total sugar yield. To explore the potential use of pretreated DPC, an anaerobic digestion was conducted on untreated and acid-pretreated DPC at 120 °C for 90 min. The results showed that pretreatment increased the total bioproduct yield, including hydrogen, ethanol, and volatile fatty acid yields, by 59.75%. This demonstrates the significant impact of pretreatment on product yields in a bioprocess.
摘要：
生产的日期糖浆产生大量的日期压蛋糕（DPC），纤维和保湿材料，具有通过生物加工产生价值的巨大潜力。然而,DPC的顽固结构影响生物过程中产品的产量。为了提高结构的可及性以及增加碳水化合物的可溶性部分，并促进进一步的酶水解，水热和稀酸（0.5％（v/v）硫酸）预处理作为具有成本效益和可行的方法，在相对较低的温度（80、100、120和140°C）和反应时间（60和90分钟）。然后通过使用纤维素酶和半纤维素的酶混合物的酶后处理来评估预处理的成功。基于总可获得的糖和最少产生的抑制剂，最佳操作条件被认为是在120°C下酸预处理90分钟，总糖收率提高55.02％。为了探索预处理DPC的潜在用途，在120°C下对未处理和酸预处理的DPC进行厌氧消化90分钟。结果表明，预处理提高了总生物制品产量，包括氢,乙醇,和挥发性脂肪酸产量，59.75%。这表明在生物过程中预处理对产物产率的显著影响。
