PDF(Pubmed)
Abstract：
CONCLUSIONS: A total of 544 significant marker-trait associations and 286 candidate genes associated with total protein and 18 amino acids were identified. Thirty-three candidate genes were found near the strong marker trait associations (- log10P ≥ 5.5). Pearl millet (Pennisetum glaucum) is largely grown as a subsistence crop in South Asia and sub-Saharan Africa. It serves as a major source of daily protein intake in these regions. Despite its importance, no systematic effort has been made to study the genetic variations of protein and amino acid content in pearl millet germplasm. The present study was undertaken to dissect the global genetic variations of total protein and 18 essential and non-essential amino acids in pearl millet, using a set of 435 K Single Nucleotide Polymorphisms (SNPs) and 161 genotypes of the Pearl Millet Inbred Germplasm Association Panel (PMiGAP). A total of 544 significant marker-trait associations (at P < 0.0001; - log10P ≥ 4) were detected and 23 strong marker-trait associations were identified using Bonferroni\'s correction method. Forty-eight pleiotropic loci were found in the genome for the studied traits. In total, 286 candidate genes associated with total protein and 18 amino acids were identified. Thirty-three candidate genes were found near strongly associated SNPs. The associated markers and the candidate genes provide an insight into the genetic architecture of the traits studied and are going to be useful in breeding improved pearl millet varieties in the future. Availabilities of improved pearl millet varieties possessing higher protein and amino acid compositions will help combat the rising malnutrition problem via diet.
摘要：
结论：总共鉴定了544个显著的标记-性状关联和286个与总蛋白和18个氨基酸相关的候选基因。在强标记性状关联（-log10P≥5.5）附近发现了33个候选基因。珍珠粟（狼尾草）在南亚和撒哈拉以南非洲地区主要作为一种自给作物种植。它是这些地区每日蛋白质摄入量的主要来源。尽管它很重要,珍珠谷子种质中蛋白质和氨基酸含量的遗传变异尚未进行系统的研究。本研究旨在剖析珍珠小米中总蛋白和18种必需和非必需氨基酸的全球遗传变异，使用一组435K单核苷酸多态性（SNP）和161个基因型的珍珠小米近交系种质关联小组（PMiGAP）。使用Bonferroni的校正方法，共检测到544个显着的标记-性状关联（P<0.0001；-log10P≥4），并鉴定了23个强标记-性状关联。在基因组中发现了48个多效性位点，用于研究性状。总的来说,鉴定了286个与总蛋白和18个氨基酸相关的候选基因。在强相关的SNP附近发现了33个候选基因。相关的标记和候选基因提供了对所研究性状的遗传结构的见解，并将在将来育种改良的珍珠小米品种中有用。具有更高蛋白质和氨基酸组成的改良珍珠小米品种的有效性将有助于通过饮食对抗日益严重的营养不良问题。
