PDF(Pubmed)
Abstract：
Brain-derived neurotrophic factor (BDNF) is a crucial mediator of neuronal plasticity. Here, we investigated the effects of controlled normobaric hypoxia (NH) combined with physical inactivity on BDNF blood levels and executive functions. A total of 25 healthy adults (25.8 ± 3.3 years, 15 female) were analyzed in a randomized controlled cross-over study. Each intervention began with a 30 min resting phase under normoxia (NOR), followed by a 90 min continuation of NOR or NH (peripheral oxygen saturation [SpO2] 85-80%). Serum and plasma samples were collected every 15 min. Heart rate and SpO2 were continuously measured. Before and after each exposure, cognitive tests were performed and after 24 h another follow-up blood sample was taken. NH decreased SpO2 (p < 0.001, ηp2 = 0.747) and increased heart rate (p = 0.006, ηp2 = 0.116) significantly. The 30-min resting phase under NOR led to a significant BDNF reduction in serum (p < 0.001, ηp2 = 0.581) and plasma (p < 0.001, ηp2 = 0.362). Continuation of NOR further significantly reduced BDNF after another 45 min (p = 0.018) in serum and after 30 min (p = 0.040) and 90 min (p = 0.005) in plasma. There was no significant BDNF decline under NH. A 24 h follow-up examination showed a significant decline in serum BDNF, both after NH and NOR. Our results show that NH has the potential to counteract physical inactivity-induced BDNF decline. Therefore, our study emphasizes the need for a physically active lifestyle and its positive effects on BDNF. This study also demonstrates the need for a standardized protocol for future studies to determine BDNF in serum and plasma.
摘要：
脑源性神经营养因子（BDNF）是神经元可塑性的关键介质。这里,我们研究了控制性常压低氧（NH）联合体力活动对BDNF血液水平和执行功能的影响。共25名健康成人(25.8±3.3岁,在随机对照交叉研究中分析了15名女性）。每次干预都始于常氧(NOR)下30分钟的静息期，随后持续90分钟的NOR或NH（外周血氧饱和度[SpO2]85-80％）。每15分钟收集血清和血浆样品。连续测量心率和SpO2。在每次曝光之前和之后，我们进行了认知测试,24小时后再进行一次血样随访.NH显著降低SpO2(p<0.001,ηp2=0.747),增加心率(p=0.006,ηp2=0.116)。在NOR下30分钟的静息阶段导致血清（p<0.001，ηp2=0.581）和血浆（p<0.001，ηp2=0.362）中BDNF的显着减少。在血清中的另外45分钟（p=0.018）之后以及在血浆中的30分钟（p=0.040）和90分钟（p=0.005）之后，NOR的持续进一步显着降低了BDNF。NH下BDNF无显著下降。24h随访检查显示血清BDNF显著下降，在NH和NOR之后。我们的结果表明，NH有可能抵消体力活动不足引起的BDNF下降。因此,我们的研究强调了身体活跃的生活方式的必要性及其对BDNF的积极影响.该研究还表明需要用于未来研究的标准化方案来确定血清和血浆中的BDNF。
