Abstract：
Traumatic brain injury (TBI) is associated with the etiology of multiple neurological disorders, including neurodegeneration, leading to various cognitive deficits. Daidzin (obtained from kudzu root and soybean leaves) is known for its neuroprotective effects through multiple mechanisms. This study aimed to investigate the pharmacological effects of Daidzin on sensory, and biochemical parameters, cognitive functions, anxiety, and depressive-like behaviors in the TBI rat model. Rats were divided into four groups (Control, TBI, TBI + Ibuprofen (30 mg/kg), and TBI + Daidzin (5 mg/kg)). Rats were subjected to TBI by dropping a 200 g rod from a height of 26 cm, resulting in an impact force of 0.51 J on the exposed crania. Ibuprofen (30 mg/kg) was used as a positive control reference/standard drug and Daidzin (5 mg/kg) as the test drug. Neurological severity score (NSS) assessment was done to determine the intactness of sensory and motor responses. Brain tissue edema and acetylcholine levels were determined in the cortex and hippocampus. Cognitive functions such as hippocampus-dependent memory, novel object recognition, exploration, depressive and anxiety-like behaviors were measured. Treatment with Daidzin improved NSS, reduced hippocampal and cortical edema, and improved levels of acetylcholine in TBI-induced rats. Furthermore, Daidzin treatment improved hippocampus-dependent memory, exploration behavior, and novel object recognition while reducing depressive and anxiety-like behavior. Our study revealed that Daidzin has a therapeutic potential comparable to Ibuprofen and can offer neuroprotection and enhanced cognitive and behavioral outcomes in rats after TBI.
摘要：
创伤性脑损伤（TBI）与多种神经系统疾病的病因有关，包括神经变性,导致各种认知缺陷。Daidzin（从葛根和大豆叶获得）以其通过多种机制的神经保护作用而闻名。本研究旨在探讨Daidzin对感官的药理作用,和生化参数，认知功能，焦虑,和TBI大鼠模型中的抑郁样行为。大鼠分为四组(对照组，TBI,TBI+布洛芬（30mg/kg），和TBI+Daidzin(5mg/kg)。通过从26cm的高度放下200g棒，对大鼠进行TBI，对裸露的颅骨产生0.51J的冲击力。布洛芬(30mg/kg)用作阳性对照参考/标准药物,Daidzin(5mg/kg)用作测试药物。进行神经系统严重程度评分（NSS）评估以确定感觉和运动反应的完整性。在皮质和海马中确定脑组织水肿和乙酰胆碱水平。认知功能，如海马依赖性记忆，新颖的物体识别，探索,测量抑郁和焦虑样行为。Daidzin治疗改善了NSS，海马和皮质水肿减少，并改善TBI诱导大鼠的乙酰胆碱水平。此外，Daidzin治疗改善海马依赖性记忆，探索行为，和新颖的物体识别，同时减少抑郁和焦虑样行为。我们的研究表明，Daidzin具有与布洛芬相当的治疗潜力，并且可以在TBI后的大鼠中提供神经保护和增强的认知和行为结果。
