PDF(Pubmed)
Abstract：
OBJECTIVE: To test the use of rainbow trout skin as a surgical mesh in abdominal hernioplasties in rats.
METHODS: The experiment involved 20 Wistar rats receiving implants of trout skin processed for disinfection in 0.5% glutaraldehyde and preserved in 100% glycerin. The animals were divided into four groups, divided at 7, 15, 30, and 90 days postoperatively. Clinical and infrared thermography evaluations were performed, and after euthanasia, assessments of adhesion formations and sample collection for histological evaluation were conducted.
RESULTS: The implant was observed to be intact, ensuring the integrity of the abdominal wall, support for the viscera, and normal mobility for the rats for up to 90 days. Low rates of clinical alterations were observed, with an intense inflammatory reaction up to day 7, chronic inflammation and the onset of angiogenesis at day 15, and a low inflammatory reaction with collagenous infiltrate and fibrosis at day 30. At day 90, the implants showed a collagenous and fibrotic infiltrate with a minimal inflammatory infiltrate.
CONCLUSIONS: The surgical mesh of trout skin performed well, making it a potential alternative for surgical procedures in muscle aponeurotic corrections in the abdominal wall.
METHODS: The experiment involved 20 Wistar rats receiving implants of trout skin processed for disinfection in 0.5% glutaraldehyde and preserved in 100% glycerin. The animals were divided into four groups, divided at 7, 15, 30, and 90 days postoperatively. Clinical and infrared thermography evaluations were performed, and after euthanasia, assessments of adhesion formations and sample collection for histological evaluation were conducted.
RESULTS: The implant was observed to be intact, ensuring the integrity of the abdominal wall, support for the viscera, and normal mobility for the rats for up to 90 days. Low rates of clinical alterations were observed, with an intense inflammatory reaction up to day 7, chronic inflammation and the onset of angiogenesis at day 15, and a low inflammatory reaction with collagenous infiltrate and fibrosis at day 30. At day 90, the implants showed a collagenous and fibrotic infiltrate with a minimal inflammatory infiltrate.
CONCLUSIONS: The surgical mesh of trout skin performed well, making it a potential alternative for surgical procedures in muscle aponeurotic corrections in the abdominal wall.
摘要：
目的：测试虹鳟鱼皮肤作为手术网片在大鼠腹部疝中的应用。
方法：实验涉及20只Wistar大鼠，接受鳟鱼皮植入物，在0.5％戊二醛中进行消毒处理，并在100％甘油中保存。这些动物被分成四组,在术后7、15、30和90天分开。进行了临床和红外热成像评估，安乐死之后,进行粘连形成的评估和用于组织学评估的样品收集。
结果：观察到植入物完好无损，确保腹壁的完整性，支持内脏，和大鼠的正常活动长达90天。观察到临床改变率低，在第7天有强烈的炎症反应，在第15天有慢性炎症和血管生成的发作，在第30天有胶原浸润和纤维化的低炎症反应。在第90天,植入物显示胶原和纤维化浸润,具有最小的炎性浸润。
结论：鳟鱼皮肤的外科网片表现良好，使其成为腹壁肌肉腱膜矫正手术的潜在替代方法。
方法：实验涉及20只Wistar大鼠，接受鳟鱼皮植入物，在0.5％戊二醛中进行消毒处理，并在100％甘油中保存。这些动物被分成四组,在术后7、15、30和90天分开。进行了临床和红外热成像评估，安乐死之后,进行粘连形成的评估和用于组织学评估的样品收集。
结果：观察到植入物完好无损，确保腹壁的完整性，支持内脏，和大鼠的正常活动长达90天。观察到临床改变率低，在第7天有强烈的炎症反应，在第15天有慢性炎症和血管生成的发作，在第30天有胶原浸润和纤维化的低炎症反应。在第90天,植入物显示胶原和纤维化浸润,具有最小的炎性浸润。
结论：鳟鱼皮肤的外科网片表现良好，使其成为腹壁肌肉腱膜矫正手术的潜在替代方法。
