PDF(Pubmed)
Abstract：
Bacterial zoonoses are established causes of severe febrile illness in East Africa. Within a fever etiology study, we applied a high-throughput 16S rRNA metagenomic assay validated for detecting bacterial zoonotic pathogens. We enrolled febrile patients admitted to 2 referral hospitals in Moshi, Tanzania, during September 2007-April 2009. Among 788 participants, median age was 20 (interquartile range 2-38) years. We performed PCR amplification of V1-V2 variable region 16S rRNA on cell pellet DNA, then metagenomic deep-sequencing and pathogenic taxonomic identification. We detected bacterial zoonotic pathogens in 10 (1.3%) samples: 3 with Rickettsia typhi, 1 R. conorii, 2 Bartonella quintana, 2 pathogenic Leptospira spp., and 1 Coxiella burnetii. One other sample had reads matching a Neoerhlichia spp. previously identified in a patient from South Africa. Our findings indicate that targeted 16S metagenomics can identify bacterial zoonotic pathogens causing severe febrile illness in humans, including potential novel agents.
摘要：
细菌性人畜共患病是东非严重发热疾病的确定原因。在发烧病因研究中，我们应用了高通量16SrRNA宏基因组检测方法,该方法验证了细菌人畜共患病原体的检测.我们招募了在莫希的2家转诊医院住院的发热患者，坦桑尼亚,2007年9月至2009年4月。在788名参与者中，中位年龄为20岁(四分位距2-38岁).我们对细胞沉淀DNA进行了V1-V2可变区16SrRNA的PCR扩增，然后进行宏基因组深度测序和病原分类鉴定。我们在10个（1.3％）样本中检测到细菌人畜共患病病原体：3个带有伤寒立克次体，1R.Conorii，2巴尔通菌，2致病性钩端螺旋体。,和1个伯氏柯西拉。另一个样品具有与Neoerhlichiaspp匹配的读数。先前在一名来自南非的患者中发现。我们的发现表明，有针对性的16S宏基因组学可以鉴定引起人类严重发热疾病的细菌人畜共患病原体，包括潜在的新代理人。
