PDF(Pubmed)
Abstract：
The auxin/indoleacetic acid (Aux/IAA) family plays a central role in regulating gene expression during auxin signal transduction. Nonetheless, there is limited knowledge regarding this gene family in sugarcane. In this study, 92 members of the IAA family were identified in Saccharum spontaneum, distributed on 32 chromosomes, and classified into three clusters based on phylogeny and motif compositions. Segmental duplication and recombination events contributed largely to the expansion of this superfamily. Additionally, cis-acting elements in the promoters of SsIAAs involved in plant hormone regulation and stress responsiveness were predicted. Transcriptomics data revealed that most SsIAA expressions were significantly higher in stems and basal parts of leaves, and at nighttime, suggesting that these genes might be involved in sugar transport. QRT-PCR assays confirmed that cold and salt stress significantly induced four and five SsIAAs, respectively. GFP-subcellular localization showed that SsIAA23 and SsIAA12a were localized in the nucleus, consistent with the results of bioinformatics analysis. In conclusion, to a certain extent, the functional redundancy of family members caused by the expansion of the sugarcane IAA gene family is related to stress resistance and regeneration of sugarcane as a perennial crop. This study reveals the gene evolution and function of the SsIAA gene family in sugarcane, laying the foundation for further research on its mode of action.
摘要：
生长素/吲哚乙酸（Aux/IAA）家族在生长素信号转导过程中调节基因表达中起着核心作用。尽管如此，关于甘蔗这个基因家族的知识有限。在这项研究中,在糖精中鉴定出IAA家族的92名成员，分布在32条染色体上，并根据系统发育和基序组成分为三个簇。分段复制和重组事件在很大程度上促进了该超家族的扩展。此外,预测了参与植物激素调节和胁迫反应的SsIAAs启动子中的顺式作用元件。转录组学数据显示，大多数SsIAA在茎和叶的基部表达显着升高，在夜间,表明这些基因可能参与糖的运输。QRT-PCR检测证实，冷胁迫和盐胁迫显著诱导了4种和5种SsIAAs，分别。GFP亚细胞定位显示SsIAA23和SsIAA12a定位于细胞核,与生物信息学分析结果一致。总之,在某种程度上,甘蔗IAA基因家族的扩展引起的家族成员的功能冗余与甘蔗作为多年生作物的抗逆性和再生有关。本研究揭示了甘蔗SsIAA基因家族的基因进化和功能,为进一步研究其作用方式奠定基础。
