背景:甘蔗中蔗糖的积累受多种环境和遗传因素的影响,植物水分对于其在甘蔗茎中糖的合成和运输中的作用至关重要,影响蔗糖浓度。总的来说,成熟期降雨和高土壤湿度促进植物生长,在哥伦比亚潮湿地区增加鲜重并降低蔗糖产量。因此,本研究旨在通过全基因组关联研究(GWAS),鉴定哥伦比亚潮湿环境中与蔗糖积累或生产相关的标记.
结果:在种植后的10个月(早熟)和13个月(正常成熟),对来自Cenicaña的不同面板的220个基因型进行了蔗糖浓度测量。对于早熟数据是在植物甘蔗和第一次再生期间收集的,而在正常成熟时,它是在植物甘蔗期间,首先,和第二个ratoon。通过GBS对220个基因型进行测序后,总共选择了137,890个SNP,RADSeq,和全基因组测序。经过GWAS分析,共有77个标记与两个年龄段的蔗糖浓度显着相关,但是只有39个接近先前报道的蔗糖积累和/或生产的候选基因。在候选基因中,强调了18个,因为它们参与了蔗糖水解(SUS6,CIN3,CINV1,CINV2),糖运输(即,MST1、MST2、PLT5、SUT4、ERD6等),磷酸化过程(TPS基因),糖酵解(PFP-ALPHA,HXK3、PHI1)、和转录因子(ERF12,ERF112)。同样,64个基因与糖基转移酶相关,糖苷酶,和荷尔蒙。
结论:这些结果为甘蔗蔗糖积累的分子机制提供了新的见解,并为哥伦比亚潮湿环境中的未来研究提供了重要的基因组资源。同样,鉴定的标记将在Cenicaña的育种计划中进行验证,以帮助育种种群的发展。
BACKGROUND: Sucrose accumulation in sugarcane is affected by several environmental and genetic factors, with plant moisture being of critical importance for its role in the synthesis and transport of sugars within the cane stalks, affecting the sucrose concentration. In general, rainfall and high soil humidity during the ripening stage promote plant growth, increasing the fresh weight and decreasing the sucrose yield in the humid region of Colombia. Therefore, this study aimed to identify markers associated with sucrose accumulation or production in the humid environment of Colombia through a genome-wide association study (GWAS).
RESULTS: Sucrose concentration measurements were taken in 220 genotypes from the Cenicaña\'s diverse panel at 10 (early maturity) and 13 (normal maturity) months after planting. For early maturity data was collected during plant cane and first ratoon, while at normal maturity it was during plant cane, first, and second ratoon. A total of 137,890 SNPs were selected after sequencing the 220 genotypes through GBS, RADSeq, and whole-genome sequencing. After GWAS analysis, a total of 77 markers were significantly associated with sucrose concentration at both ages, but only 39 were close to candidate genes previously reported for sucrose accumulation and/or production. Among the candidate genes, 18 were highlighted because they were involved in sucrose hydrolysis (SUS6, CIN3, CINV1, CINV2), sugar transport (i.e., MST1, MST2, PLT5, SUT4, ERD6 like), phosphorylation processes (TPS genes), glycolysis (PFP-ALPHA, HXK3, PHI1), and transcription factors (ERF12, ERF112). Similarly, 64 genes were associated with glycosyltransferases, glycosidases, and hormones.
CONCLUSIONS: These results provide new insights into the molecular mechanisms involved in sucrose accumulation in sugarcane and contribute with important genomic resources for future research in the humid environments of Colombia. Similarly, the markers identified will be validated for their potential application within Cenicaña\'s breeding program to assist the development of breeding populations.