PDF(Pubmed)
Abstract：
In response to biotic and abiotic stresses, the WRKY gene family plays a crucial role in plant growth and development. This study focused on Phoebe bournei and involved genome-wide identification of WRKY gene family members, clarification of their molecular evolutionary characteristics, and comprehensive mapping of their expression profiles under diverse abiotic stress conditions. A total of 60 WRKY gene family members were identified, and their phylogenetic classification revealed three distinct groups. A conserved motif analysis underscored the significant conservation of motif 1 and motif 2 among the majority of PbWRKY proteins, with proteins within the same class sharing analogous gene structures. Furthermore, an examination of cis-acting elements and protein interaction networks revealed several genes implicated in abiotic stress responses in P. bournei. Transcriptomic data were utilized to analyze the expression patterns of WRKY family members under drought and waterlogged conditions, with subsequent validation by quantitative real-time PCR (RT-qPCR) experiments. Notably, PbWRKY55 exhibited significant expression modulation under drought stress; PbWRKY36 responded prominently to waterlogging stress; and PbWRKY18, PbWRKY38, and PbWRKY57 demonstrated altered expression under both drought and waterlogging stresses. This study revealed the PbWRKY candidate genes that potentially play a pivotal role in enhancing abiotic stress resilience in P. bournei. The findings have provided valuable insights and knowledge that can guide further research aimed at understanding and addressing the impacts of abiotic stress within this species.
摘要：
为了应对生物和非生物胁迫，WRKY基因家族在植物生长发育中起着至关重要的作用。本研究集中于Phoebebournei，涉及WRKY基因家族成员的全基因组鉴定，阐明它们的分子进化特征，以及在不同非生物胁迫条件下它们的表达谱的全面作图。共鉴定出60个WRKY基因家族成员,它们的系统发育分类揭示了三个不同的群体。保守的基序分析强调了基序1和基序2在大多数PbWRKY蛋白中的显著保守性,同一类蛋白质共享类似的基因结构。此外，对顺式作用元件和蛋白质相互作用网络的研究揭示了几个基因与P.bournei的非生物胁迫反应有关。转录组数据用于分析WRKY家族成员在干旱和淹水条件下的表达模式。随后通过定量实时PCR（RT-qPCR）实验进行验证。值得注意的是,PbWRKY55在干旱胁迫下表现出显着的表达调节；PbWRKY36对淹水胁迫反应显着；在干旱和淹水胁迫下，PbWRKY18，PbWRKY38和PbWRKY57表现出表达变化。这项研究揭示了PbWRKY候选基因，这些基因可能在增强P.bournei的非生物胁迫恢复力中起关键作用。这些发现提供了宝贵的见解和知识，可以指导旨在理解和解决该物种非生物胁迫影响的进一步研究。
