GATA转录因子是调节转录的关键蛋白质,其特征在于IV型锌指DNA结合域。它们在植物的生长和发育中起着重要作用。虽然GATA家族基因已经在几种植物物种中被鉴定出来,菲比·伯恩尼还没有报道。在这项研究中,从鲍尔内疟原虫基因组中鉴定出22个GATA家族基因,以及它们的物理化学性质,染色体分布,亚细胞定位,系统发育树,保守的图案,基因结构,启动子中的顺式调控元件,并分析了在植物组织中的表达。系统发育分析表明,PbGATAs明确分为四个亚科。它们在12条染色体中的11条染色体上分布不均,除了9号染色体.顺式启动子主要参与环境压力和激素调节。进一步的研究表明,PbGATA11定位于叶绿体并在五个组织中表达,包括树皮,根木质部,茎皮,茎木质部,叶,这意味着PbGATA11可能在调节叶绿素合成中具有潜在的作用。最后,四个代表性基因的表达谱,干旱下的PbGATA5、PbGATA12、PbGATA16和PbGATA22,盐度,和温度应力,通过qRT-PCR检测。结果表明,PbGATA5、PbGATA22和PbGATA16在干旱胁迫下显著表达。PbGATA12和PbGATA22在10℃低温胁迫8小时后显著表达。本研究的结论是,Pbunnei的PbGATA家族基因的生长发育对应对逆境压力至关重要。本研究为研究GATAs的演变提供了新的思路,为PbGATA基因的未来功能分析提供了有用的信息,并有助于更好地了解伯尼的非生物胁迫反应。
GATA transcription factors are crucial proteins in regulating transcription and are characterized by a type-IV zinc finger DNA-binding domain. They play a significant role in the growth and development of plants. While the GATA family gene has been identified in several plant species, it has not yet been reported in Phoebe bournei. In this study, 22 GATA family genes were identified from the P. bournei genome, and their physicochemical properties, chromosomal distribution, subcellular localization, phylogenetic tree, conserved motif, gene structure, cis-regulatory elements in promoters, and expression in plant tissues were analyzed. Phylogenetic analysis showed that the PbGATAs were clearly divided into four subfamilies. They are unequally distributed across 11 out of 12 chromosomes, except chromosome 9. Promoter cis-elements are mostly involved in environmental stress and hormonal regulation. Further studies showed that PbGATA11 was localized to chloroplasts and expressed in five tissues, including the root bark, root xylem, stem bark, stem xylem, and leaf, which means that PbGATA11 may have a potential role in the regulation of chlorophyll synthesis. Finally, the expression profiles of four representative genes, PbGATA5, PbGATA12, PbGATA16, and PbGATA22, under drought, salinity, and temperature stress, were detected by qRT-PCR. The results showed that PbGATA5, PbGATA22, and PbGATA16 were significantly expressed under drought stress. PbGATA12 and PbGATA22 were significantly expressed after 8 h of low-temperature stress at 10 °C. This study concludes that the growth and development of the PbGATA family gene in P. bournei in coping with adversity stress are crucial. This study provides new ideas for studying the evolution of GATAs, provides useful information for future functional analysis of PbGATA genes, and helps better understand the abiotic stress response of P. bournei.