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Abstract：
This study presents properties of hydroethanolic extracts prepared from Pinot Noir (PN) grape pomace through conventional, ultrasound-assisted or solvothermal extraction. The components of the extracts were identified by HPLC. The total content of polyphenols, flavonoids, anthocyanins, and condensed tannins, as well as antioxidant activity and α-glucosidase inhibitory activity of extracts were evaluated using UV-vis spectroscopy. All extracts were rich in phenolic compounds, proving a good radical scavenging activity. The extract obtained by conventional extraction at 80 °C showed the best α-glucosidase inhibitory activity close to that of (-)-epigallocatechin gallate. To improve the chemical stability of polyphenols, the chosen extract was incorporated in porous silica-based supports: amine functionalized silica (MCM-NH2), fucoidan-coated amine functionalized silica (MCM-NH2-Fuc), MCM-41, and diatomite. The PN extract exhibited moderate activity against Gram-positive S. aureus (MIC = 156.25 μg/mL) better than against Gram-negative E. coli (MIC = 312.5 μg/mL). The biocompatibility of PN extract, free and incorporated in MCM-NH2 and MCM-NH2-Fuc, was assessed on RAW 264.7 mouse macrophage cells, and the samples showcased a good cytocompatibility at 10 µg/mL concentration. At this concentration, PN and PN@MCM-NH2-Fuc reduced the inflammation by inhibiting NO production. The anti-inflammatory potential against COX and LOX enzymes of selected samples was evaluated and compared with that of Indomethacin and Zileuton, respectively. The best anti-inflammatory activity was observed when PN extract was loaded on MCM-NH2-Fuc support.
摘要：
本研究介绍了通过常规方法从黑皮诺（PN）葡萄果渣中制备的乙醇提取物的特性，超声辅助或溶剂热提取。通过HPLC鉴定提取物的组分。多酚的总含量，黄酮类化合物，花青素,和浓缩的单宁，使用UV-vis光谱法评估提取物的抗氧化活性和α-葡萄糖苷酶抑制活性。所有提取物都富含酚类化合物，证明了良好的自由基清除活性。通过在80°C下常规提取获得的提取物显示出与（-）-表没食子儿茶素没食子酸酯接近的最佳α-葡萄糖苷酶抑制活性。为了提高多酚的化学稳定性，选择的提取物被掺入多孔二氧化硅基载体:胺官能化二氧化硅(MCM-NH2)，岩藻依聚糖包覆的胺官能化二氧化硅（MCM-NH2-Fuc），MCM-41和硅藻土。PN提取物对革兰氏阳性金黄色葡萄球菌(MIC=156.25μg/mL)表现出优于对革兰氏阴性大肠杆菌(MIC=312.5μg/mL)的中等活性。PN提取物的生物相容性，游离并掺入MCM-NH2和MCM-NH2-Fuc，在RAW264.7小鼠巨噬细胞上进行评估，样品在10µg/mL浓度下显示出良好的细胞相容性。在这个浓度下，PN和PN@MCM-NH2-Fuc通过抑制NO产生减少炎症。评估了选定样品的抗COX和LOX酶的抗炎潜力，并将其与吲哚美辛和齐留通进行了比较。分别。当PN提取物负载在MCM-NH2-Fuc支持物上时,观察到最好的抗炎活性。
