PDF(Pubmed)
Abstract：
Recent studies have exhibited a very promising role of copper nanoparticles (CuNPs) in mitigation of abiotic stresses in plants. Arbuscular mycorrhizae fungi (AMF) assisted plants to trigger their defense mechanism against abiotic stresses. Arsenic (As) is a non-essential and injurious heavy-metal contaminant. Current research work was designed to elucidate role of CuNPs (100, 200 and 300 mM) and a commercial inoculum of Glomus species (Clonex® Root Maximizer) either alone or in combination (CuNPs + Clonex) on physiology, growth, and stress alleviation mechanisms of E. sibiricus growing in As spiked soils (0, 50, and 100 mg Kg- 1 soil). Arsenic induced oxidative stress, enhanced biosynthesis of hydrogen peroxide, lipid peroxidation and methylglyoxal (MG) in E. sibiricus. Moreover, As-phytotoxicity reduced photosynthetic activities and growth of plants. Results showed that individual and combined treatments, CuNPs (100 mM) as well as soil inoculation of AMF significantly enhanced root growth and shoot growth by declining As content in root tissues and shoot tissues in As polluted soils. E. sibiricus plants treated with CuNPs (100 mM) and/or AMF alleviated As induced phytotoxicity through upregulating the activity of antioxidative enzymes such as catalase (CAT) and superoxide dismutase (SOD) besides the biosynthesis of non-enzymatic antioxidants including phytochelatin (PC) and glutathione (GSH). In brief, supplementation of CuNPs (100 mM) alone or in combination with AMF reduced As uptake and alleviated the As-phytotoxicity in E. sibiricus by inducing stress tolerance mechanism resulting in the improvement of the plant growth parameters.
摘要：
最近的研究表明，铜纳米颗粒（CuNPs）在减轻植物非生物胁迫方面具有非常有希望的作用。丛枝菌根真菌（AMF）辅助植物触发其对非生物胁迫的防御机制。砷（As）是一种非必需且有害的重金属污染物。当前的研究工作旨在阐明CuNPs（100、200和300mM）和Glomus物种的商业接种物（Clonex®RootMaximizer）单独或组合（CuNPsClonex）在生理学上的作用。增长,和在加标土壤(0、50和100毫克千克-1土壤)中生长的sibiricus的胁迫缓解机制。砷诱导的氧化应激，增强过氧化氢的生物合成，E.sibiricus的脂质过氧化和甲基乙二醛（MG）。此外，植物毒性降低了植物的光合活性和生长。结果表明，单独和联合治疗，CuNPs（100mM）以及土壤接种AMF通过降低As污染土壤中根组织和芽组织中As的含量，显着提高了根的生长和芽的生长。用CuNPs(100mM)和/或AMF处理的sibiricus植物通过上调抗氧化酶如过氧化氢酶(CAT)和超氧化物歧化酶(SOD)的活性,除了包括植物螯合素(PC)和谷胱甘肽(GSH)在内的非酶抗氧化剂的生物合成之外,减轻了As诱导的植物毒性。简而言之,单独或与AMF组合补充CuNPs（100mM）可通过诱导胁迫耐受性机制降低As的吸收并减轻As的植物毒性，从而改善植物生长参数。
