PDF(Pubmed)
Abstract：
In zebrafish, like in mammals, radial glial cells (RGCs) can act as neural progenitors during development and regeneration in adults. However, the heterogeneity of glia subpopulations entails the need for different specific markers of zebrafish glia. Currently, fluorescent protein expression mediated by a regulatory element from the glial fibrillary acidic protein (gfap) gene is used as a prominent glia reporter. We now expand this tool by demonstrating that a regulatory element from the mouse Fatty acid binding protein 7 (Fabp7) gene drives reliable expression in fabp7-expressing zebrafish glial cells. By using three different Fabp7 regulatory element-mediated fluorescent protein reporter strains, we reveal in double transgenic zebrafish that progenitor cells expressing fluorescent proteins driven by the Fabp7 regulatory element give rise to radial glia, oligodendrocyte progenitors, and some neuronal precursors. Furthermore, Bergmann glia represent the almost only glial population of the zebrafish cerebellum (besides a few oligodendrocytes), and the radial glia also remain in the mature cerebellum. Fabp7 regulatory element-mediated reporter protein expression in Bergmann glia progenitors suggests their origin from the ventral cerebellar proliferation zone, the ventricular zone, but not from the dorsally positioned upper rhombic lip. These new Fabp7 reporters will be valuable for functional studies during development and regeneration.
摘要：
在斑马鱼中,就像哺乳动物一样，放射状神经胶质细胞（RGC）在成人的发育和再生过程中可以充当神经祖细胞。然而,胶质细胞亚群的异质性要求斑马鱼胶质细胞需要不同的特异性标记。目前,由神经胶质纤维酸性蛋白（gfap）基因的调节元件介导的荧光蛋白表达被用作突出的神经胶质报道分子。我们现在通过证明来自小鼠脂肪酸结合蛋白7（Fabp7）基因的调节元件驱动表达fabp7的斑马鱼胶质细胞中的可靠表达来扩展该工具。通过使用三种不同的Fabp7调控元件介导的荧光蛋白报告菌株，我们在双转基因斑马鱼中揭示了由Fabp7调控元件驱动的表达荧光蛋白的祖细胞会产生放射状神经胶质，少突胶质细胞祖细胞，和一些神经元前体。此外，Bergmann胶质细胞代表斑马鱼小脑的几乎唯一的神经胶质细胞（除了少数少突胶质细胞），放射状神经胶质也保留在成熟的小脑中。Bergmann胶质祖细胞中Fabp7调控元件介导的报告蛋白表达表明它们起源于腹侧小脑增殖区，心室区,但不是从背侧的菱形上唇。这些新的Fabp7报道分子对于发育和再生期间的功能研究将是有价值的。
