乳腺癌(BC)是女性常见的恶性肿瘤。环状RNA(circularRNAs,circRNAs)已被报道在BC的发展中起关键作用;然而,环状RNA锌指RNA结合蛋白(circ_ZFR)在BC中的作用尚不清楚。
circ_ZFR的丰度,脂肪酸结合蛋白7(FABP7),和microRNA-223-3p(miR-223-3p)通过定量实时聚合酶链反应(qRT-PCR)进行测量。通过RNaseR处理验证了circ_ZFR的环状结构。细胞增殖,迁移,入侵,通过集落形成评估细胞凋亡,细胞计数试剂盒-8,Transwell,流式细胞术测定,分别。通过蛋白质印迹测定所有蛋白质水平。使用双荧光素酶报告基因测定来确认miR-223-3p与circ_ZFR或FABP7之间的关系。建立异种移植模型以了解circ_ZFR对体内BC细胞生长的影响。
在BC组织和细胞系中circ_ZFR和FABP7的表达水平较高,而miR-223-3p表达较低。在BC细胞中敲除circ_ZFR或FABP7降低增殖,迁移,入侵,和上皮间质转化(EMT),并在体外诱导细胞凋亡,而在circ_ZFR过表达的细胞中观察到相反的作用。此外,circ_ZFR可能充当miR-223-3p的海绵来调节FABP7的表达,从而促进BC细胞在体外和体内的进展。
Circ_ZFR可能作为miR-223-3p的miRNA海绵来调节FABP7,从而促进增殖,迁移,入侵,和BC细胞的EMT,抑制细胞凋亡。
Breast cancer (BC) is a common malignancy in women. Circular RNAs (circRNAs) have been reported to play a key role in the development of BC; however, the effect of circular RNA zinc finger RNA binding protein (circ_ZFR) in BC is unknown.
Abundances of circ_ZFR, fatty acid binding protein 7 (
FABP7), and microRNA-223-3p (miR-223-3p) were measured by quantitative real-time polymerase chain reaction (qRT-PCR). The circular structure of circ_ZFR was validated by RNase R treatment. Cell proliferation, migration, invasion, and apoptosis were assessed by colony formation, cell counting kit-8, Transwell, flow cytometry assays, respectively. All protein levels were determined by Western blot. Dual-luciferase reporter assay was used to confirm the relationship between miR-223-3p and circ_ZFR or
FABP7. A xenograft model was established to understand the effect of circ_ZFR on BC cell growth in vivo.
The expression levels of circ_ZFR and
FABP7 were higher in BC tissues and cell lines, whereas miR-223-3p expression was lower. Knockdown of circ_ZFR or
FABP7 in BC cells reduced proliferation, migration, invasion, and epithelial mesenchymal transition (EMT), and induced apoptosis in vitro, whereas the opposite effects were observed in circ_ZFR-overexpressed cells. Furthermore, circ_ZFR might act as a sponge for miR-223-3p to regulate
FABP7 expression, thereby promoting the progression of BC cells in vitro and in vivo.
Circ_ZFR might act as a miRNA sponge for miR-223-3p to regulate FABP7, thereby promoting proliferation, migration, invasion, and EMT of BC cells, and inhibiting cell apoptosis.