利用自切割肽在邻近标记蛋白质组学中进行定制控制。
关键词: CP: Biotechnology Golden Gate assembly T2A peptide T2A split/link TurboID glucocorticoid receptor interactomics non-structural protein 7 nucleocapsid protein proteomics proximity labeling
Mesh : Proteomics / methods Humans SARS-CoV-2 / metabolism genetics Peptides / metabolism chemistry COVID-19 / metabolism virology HEK293 Cells Receptors, Glucocorticoid / metabolism genetics chemistry Coronavirus Nucleocapsid Proteins / metabolism genetics chemistry Plasmids / genetics metabolism Mass Spectrometry / methods Phosphoproteins / metabolism genetics Protein Interaction Mapping / methods
来 源:
DOI:10.1016/j.crmeth.2024.100818
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