Abstract：
In this chapter, we report advances in tissue culture applied to Passiflora. We present reproducible protocols for somatic embryogenesis, endosperm-derived triploid production, and genetic transformation for such species knowledge generated by our research team and collaborators in the last 20 years. Our research group has pioneered the work on passion fruit somatic embryogenesis, and we directed efforts to characterize several aspects of this morphogenic pathway. Furthermore, we expanded the possibilities of understanding the molecular mechanism related to developmental phase transitions of Passiflora edulis Sims. and P. cincinnata Mast., and a transformation protocol is presented for the overexpression of microRNA156.
摘要：
在这一章中,我们报道了应用于西番莲的组织培养的进展。我们提出了体细胞胚胎发生的可重复方案，胚乳衍生的三倍体生产，以及我们的研究团队和合作者在过去20年中产生的这种物种知识的遗传转化。我们的研究小组开创了百香果体细胞胚胎发生的工作，我们致力于描述这种形态发生途径的几个方面。此外，我们扩展了理解与西番莲发育相变相关的分子机制的可能性。还有辛辛那塔桅杆.,并提出了过表达microRNA156的转化方案。
