{Reference Type}: Journal Article
{Title}: Advances in Tissue Culture and Transformation Studies in Non-model Species: Passiflora spp. (Passifloraceae).
{Author}: Otoni WC;Soares JR;Souza CS;Silva LAS;Dias LLL;Robledo KJM;Paim-Pinto DL;Koehler AD;Sodrzeieski PA;Fernandes AM;Vieira LM;Silva PO;Silveira EC;Matos EM;Carvalho IF;Romanel E;Batista DS;Viccini LF;Faleiro FG;Rocha DI;Nogueira FTS;Silva MLC;
{Journal}: Methods Mol Biol
{Volume}: 2827
{Issue}: 0
{Year}: 2024
暂无{DOI}: 10.1007/978-1-0716-3954-2_15
{Abstract}: In this chapter, we report advances in tissue culture applied to Passiflora. We present reproducible protocols for somatic embryogenesis, endosperm-derived triploid production, and genetic transformation for such species knowledge generated by our research team and collaborators in the last 20 years. Our research group has pioneered the work on passion fruit somatic embryogenesis, and we directed efforts to characterize several aspects of this morphogenic pathway. Furthermore, we expanded the possibilities of understanding the molecular mechanism related to developmental phase transitions of Passiflora edulis Sims. and P. cincinnata Mast., and a transformation protocol is presented for the overexpression of microRNA156.