Abstract：
The success of in vitro cultivation, particularly for micropropagation purposes, depends on the efficient control of contaminants. In this context, the sterilization of plant material constitutes a fundamental step in initiating cultures. Microbial contaminants can be found either on the surface (epiphyte) or inside plant explants (endophyte). However, the latter is generally challenging to detect and may not always be eradicated through surface sterilization alone. Endophyte contaminants, such as bacteria, can persist within plant material over several cultivation cycles, potentially interfering with or inhibiting in vitro establishment, growth, or recovery of cryopreserved materials. Therefore, microscopy techniques, such as electron microscopy, can yield valuable insights into bacterial endophytes\' localization, tissue colonization patterns, and functions in in vitro plant culture. This information is essential for adopting effective strategies for eliminating, preventing, or harmonious coexistence with contaminants.
摘要：
体外培养的成功,特别是为了微繁殖的目的，取决于对污染物的有效控制。在这种情况下，植物材料的灭菌是开始培养的基本步骤。微生物污染物可以在表面（附生植物）或植物外植体（内生菌）内部发现。然而,后者通常难以检测，并且可能并不总是仅通过表面灭菌来根除。内生真菌污染物,如细菌，可以在植物材料中持续几个培养周期，可能干扰或抑制体外建立，增长,或回收冷冻保存的材料。因此,显微镜技术,比如电子显微镜，可以对细菌内生菌的定位产生有价值的见解，组织定植模式，并在体外植物培养中发挥作用。这些信息对于采取有效的消除战略至关重要，预防,或与污染物和谐共存。
