PDF(Pubmed)
Abstract：
UNASSIGNED: Parasite-mediated selection is considered one of the potential mechanisms contributing to the coexistence of asexual-sexual complexes. Gibel carp (Carassius gibelio), an invasive fish species in Europe, often forms populations composed of gynogenetic and sexual specimens.
UNASSIGNED: The experimental infection was induced in gynogenetic and sexual gibel carp using eye-fluke Diplostomum pseudospathaceum (Trematoda), and the transcriptome profile of the spleen as a major immune organ in fish was analyzed to reveal the differentially expressed immunity-associated genes related to D. pseudospathaceum infection differing between gynogenetic and sexual gibel carp.
UNASSIGNED: High parasite infection was found in gynogenetic fish when compared to genetically diverse sexuals. Although metacercariae of D. pseudospathaceum are situated in an immune-privileged organ, our results show that eye trematodes may induce a host immune response. We found differential gene expression induced by eye-fluke infection, with various impacts on gynogenetic and sexual hosts, documenting for the majority of DEGs upregulation in sexuals, and downregulation in asexuals. Differences in gene regulation between gynogenetic and sexual gibel carp were evidenced in many immunity-associated genes. GO analyses revealed the importance of genes assigned to the GO terms: immune function, the Notch signaling pathway, MAP kinase tyrosine/threonine/phosphatase activity, and chemokine receptor activity. KEGG analyses revealed the importance of the genes involved in 12 immunity-associated pathways - specifically, FoxO signaling, adipocytokine signaling, TGF-beta signaling, apoptosis, Notch signaling, C-type lectin receptor signaling, efferocytosis, intestinal immune network for IgA production, insulin signaling, virion - human immunodeficiency virus, Toll-like receptor signaling, and phosphatidylinositol signaling system.
UNASSIGNED: Our study indicates the limited potential of asexual fish to cope with higher parasite infection (likely a loss of capacity to induce an effective immune response) and highlights the important role of molecular mechanisms associated with immunity for the coexistence of gynogenetic and sexual gibel carp, potentially contributing to its invasiveness.
UNASSIGNED: The experimental infection was induced in gynogenetic and sexual gibel carp using eye-fluke Diplostomum pseudospathaceum (Trematoda), and the transcriptome profile of the spleen as a major immune organ in fish was analyzed to reveal the differentially expressed immunity-associated genes related to D. pseudospathaceum infection differing between gynogenetic and sexual gibel carp.
UNASSIGNED: High parasite infection was found in gynogenetic fish when compared to genetically diverse sexuals. Although metacercariae of D. pseudospathaceum are situated in an immune-privileged organ, our results show that eye trematodes may induce a host immune response. We found differential gene expression induced by eye-fluke infection, with various impacts on gynogenetic and sexual hosts, documenting for the majority of DEGs upregulation in sexuals, and downregulation in asexuals. Differences in gene regulation between gynogenetic and sexual gibel carp were evidenced in many immunity-associated genes. GO analyses revealed the importance of genes assigned to the GO terms: immune function, the Notch signaling pathway, MAP kinase tyrosine/threonine/phosphatase activity, and chemokine receptor activity. KEGG analyses revealed the importance of the genes involved in 12 immunity-associated pathways - specifically, FoxO signaling, adipocytokine signaling, TGF-beta signaling, apoptosis, Notch signaling, C-type lectin receptor signaling, efferocytosis, intestinal immune network for IgA production, insulin signaling, virion - human immunodeficiency virus, Toll-like receptor signaling, and phosphatidylinositol signaling system.
UNASSIGNED: Our study indicates the limited potential of asexual fish to cope with higher parasite infection (likely a loss of capacity to induce an effective immune response) and highlights the important role of molecular mechanisms associated with immunity for the coexistence of gynogenetic and sexual gibel carp, potentially contributing to its invasiveness.
摘要：
■寄生虫介导的选择被认为是促成无性-性复合物共存的潜在机制之一。吉贝尔鲤鱼（Carassiusgibelio），欧洲的一种入侵鱼类，通常形成由雌激素和性标本组成的种群。
■实验感染是使用眼吸虫双足口假球（吸虫）在雌核发育和有性的gibel鱼中诱导的，并分析了脾脏作为鱼类主要免疫器官的转录组特征，以揭示与雌核发育和有性gibel鱼感染有关的差异表达的免疫相关基因。
■与遗传多样性的性别相比，在雌核发育鱼类中发现了高寄生虫感染。尽管假球D.pseudospathaceum的cer虫位于免疫特权器官中，我们的研究结果表明眼吸虫可以诱导宿主的免疫反应。我们发现眼吸虫感染诱导的差异基因表达，对雌激素和性宿主有各种影响，记录大多数DEGs在性行为中的上调，以及对无性者的下调。在许多与免疫相关的基因中证明了雌核发育和有性gibel鱼之间基因调控的差异。GO分析揭示了分配给GO术语的基因的重要性：免疫功能，Notch信号通路,MAP激酶酪氨酸/苏氨酸/磷酸酶活性，和趋化因子受体活性。KEGG分析揭示了参与12种免疫相关途径的基因的重要性-特别是，FoxO信号,脂肪细胞因子信号传导，TGF-β信号,凋亡，陷波信号,C型凝集素受体信号，红细胞增多症,产生IgA的肠道免疫网络，胰岛素信号，病毒体-人类免疫缺陷病毒，Toll样受体信号,和磷脂酰肌醇信号系统。
■我们的研究表明，无性鱼应对更高寄生虫感染的潜力有限（可能是诱导有效免疫反应的能力丧失），并强调了与免疫相关的分子机制在雌核发育和有性gibel鱼共存中的重要作用，可能有助于其侵入性。
■实验感染是使用眼吸虫双足口假球（吸虫）在雌核发育和有性的gibel鱼中诱导的，并分析了脾脏作为鱼类主要免疫器官的转录组特征，以揭示与雌核发育和有性gibel鱼感染有关的差异表达的免疫相关基因。
■与遗传多样性的性别相比，在雌核发育鱼类中发现了高寄生虫感染。尽管假球D.pseudospathaceum的cer虫位于免疫特权器官中，我们的研究结果表明眼吸虫可以诱导宿主的免疫反应。我们发现眼吸虫感染诱导的差异基因表达，对雌激素和性宿主有各种影响，记录大多数DEGs在性行为中的上调，以及对无性者的下调。在许多与免疫相关的基因中证明了雌核发育和有性gibel鱼之间基因调控的差异。GO分析揭示了分配给GO术语的基因的重要性：免疫功能，Notch信号通路,MAP激酶酪氨酸/苏氨酸/磷酸酶活性，和趋化因子受体活性。KEGG分析揭示了参与12种免疫相关途径的基因的重要性-特别是，FoxO信号,脂肪细胞因子信号传导，TGF-β信号,凋亡，陷波信号,C型凝集素受体信号，红细胞增多症,产生IgA的肠道免疫网络，胰岛素信号，病毒体-人类免疫缺陷病毒，Toll样受体信号,和磷脂酰肌醇信号系统。
■我们的研究表明，无性鱼应对更高寄生虫感染的潜力有限（可能是诱导有效免疫反应的能力丧失），并强调了与免疫相关的分子机制在雌核发育和有性gibel鱼共存中的重要作用，可能有助于其侵入性。
