关键词: Biology Fly Immature Post-mortem interval Pupa

Mesh : Animals Forensic Entomology Sarcophagidae / growth & development Pupa / growth & development Temperature Brazil Cadaver Humans

来  源:   DOI:10.1038/s41598-024-65070-9   PDF(Pubmed)

Abstract:
The family Sarcophagidae is very diverse in Brazil. Due to their living habits, they are the subject of many medical, veterinary, sanitary, and entomological studies. However, Sarcophagidae species are still poorly studied in forensic entomology, although they are frequently reported in carcasses and even human corpses. Thus, this study aims to identify and compare the developmental stages and intrapuparial morphological characteristics of Peckia (Euboettcheria) collusor to serve as an auxiliary tool in forensic entomology. The pupae collected after zero hour at 27 °C and 32 °C were sacrificed every three hours until the first 24 h and then every six hours until the emergence of the first adults, using 30 pupae each time, totaling 1560 for 27 °C and 1290 for 32 °C. The intrapuparial development time of this fly species under laboratory-controlled conditions was 288 h at 27 °C and 228 h at 32 °C. The 2820 pupae were analyzed according to temperature and classified into eight possible stages. This contributed to the selection of 16 key morphological characteristics to identify the age of the pupae. The identified intrapupal morphological characteristics have great potential to help researchers, experts, technical assistants, and forensic entomologists estimate the minimum post-mortem interval (minPMI) of cadavers.
摘要:
在巴西,Sarcophagidae家族非常多样化。由于他们的生活习惯,它们是许多医学的主题,兽医,卫生,和昆虫学研究。然而,在法医昆虫学中仍然很少研究,尽管它们经常在尸体甚至人类尸体中被报道。因此,本研究旨在鉴定和比较Peckia(Euboettcheria)结合法的发育阶段和内形态特征,以作为法医昆虫学的辅助工具。在27°C和32°C零小时后收集的p每三个小时处死一次,直到第一个24小时,然后每六个小时处死一次,直到第一个成虫出现,每次使用30只蛹,27°C总计1560,32°C总计1290。在实验室控制的条件下,这种苍蝇的体内发育时间在27°C下为288小时,在32°C下为228小时。根据温度对2820只p进行了分析,并将其分为八个可能的阶段。这有助于选择16个关键形态特征来识别the的年龄。确定的盘内形态特征有很大的潜力,以帮助研究人员,专家,技术助理,法医昆虫学家估计尸体的最小死后间隔(minPMI)。
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