Abstract：
AbstractGulf of Maine waters are warming rapidly, prompting a reevaluation of how commercially important marine species will respond. The goal of this study was to determine the respiratory, cardiac, and locomotory responses of American lobsters (Homarus americanus) to increasing water temperatures and to compare these to similar published studies. First, we measured the heart rate and ventilation rate of 10 lobsters that were confined in a temperature-controlled chamber while exposing them to gradually warming temperatures from 16 to 30 °C over 7 h. Both heart rate and ventilation rate increased along with the temperature up to a break point, with the mean heart rate peaking at 26.5 ± 1.6 °C, while the ventilation rate peaked at 27.4 ± 0.8 °C. In a subset of these trials (n = 5), oxygen consumption was also monitored and peaked at similar temperatures. In a second experiment, both the heart rate and activity of five lobsters were monitored with custom-built dataloggers while they moved freely in a large tank, while the temperature was increased from 18 to 29 °C over 24 h. The heart rate of these lobsters also increased with temperature, but their initial heart rates were lower than we recorded from confined lobsters. Finally, we confirmed that the low heart rates of the freely moving lobsters were due to the methods used by comparing heart rate data from eight lobsters collected using both methods with each individual animal. Thus, while our overall results are consistent with data from previous studies, they also show that the methods used in studies of physiological and behavioral responses to warming temperatures can impact the results obtained.
摘要：
缅因州海湾水域迅速变暖，促使人们重新评估具有商业意义的海洋物种的反应。这项研究的目的是确定呼吸，心脏,以及美国龙虾（Homarusamericanus）对水温升高的运动反应，并将其与类似的已发表研究进行比较。首先,我们测量了10只龙虾的心率和通气率，这些龙虾被限制在温度控制室中，同时在7小时内将它们暴露于从16到30°C的逐渐变暖的温度下。平均心率在26.5±1.6°C达到峰值，而通风率在27.4±0.8°C达到峰值。在这些试验的子集(n=5)中，也监测了耗氧量，并在类似温度下达到峰值。在第二个实验中,五只龙虾的心率和活动都用定制的数据记录器监测，同时它们在一个大水箱中自由移动，而温度在24小时内从18°C升高到29°C。这些龙虾的心率也随着温度的升高而升高，但是他们的初始心率低于我们从封闭龙虾中记录的心率。最后,我们证实,自由活动龙虾的心率较低是由于所使用的方法是将使用两种方法收集的8只龙虾的心率数据与每只动物进行比较.因此，虽然我们的总体结果与以前研究的数据一致，他们还表明，在对升温温度的生理和行为反应的研究中使用的方法会影响所获得的结果。
