Abstract：
Skeletal muscle is one of the largest tissues in human body. Besides enabling voluntary movements and maintaining body\'s metabolic homeostasis, skeletal muscle is also a target of many pathological conditions. Mitochondria occupy 10-15% volume of a muscle myofiber and regulate many cellular processes, which often determine the fate of the cell. Isolation of mitochondria from skeletal muscle provides opportunities for various multi-omics studies with a focus on mitochondria in biomedical research field. Here we describe a protocol to efficiently isolate mitochondria with high quality and purity from skeletal muscle of mice using Nycodenz density gradient ultracentrifugation.
摘要：
骨骼肌是人体最大的组织之一。除了使自愿运动和维持身体的代谢稳态，骨骼肌也是许多病理状况的目标。线粒体占据肌肉肌纤维的10-15％体积，并调节许多细胞过程，这往往决定了细胞的命运。从骨骼肌中分离线粒体为各种多组学研究提供了机会，重点是生物医学研究领域的线粒体。在这里，我们描述了使用Nycodenz密度梯度超速离心从小鼠骨骼肌中有效分离高质量和纯度线粒体的方案。
