{Reference Type}: Journal Article
{Title}: Isolation of Mitochondria from Murine Skeletal Muscle.
{Author}: Dong L;Li X;Li A;Yi J;Zhou J;
{Journal}: Methods Mol Biol
{Volume}: 2816
{Issue}: 0
{Year}: 2024
暂无{DOI}: 10.1007/978-1-0716-3902-3_8
{Abstract}: Skeletal muscle is one of the largest tissues in human body. Besides enabling voluntary movements and maintaining body's metabolic homeostasis, skeletal muscle is also a target of many pathological conditions. Mitochondria occupy 10-15% volume of a muscle myofiber and regulate many cellular processes, which often determine the fate of the cell. Isolation of mitochondria from skeletal muscle provides opportunities for various multi-omics studies with a focus on mitochondria in biomedical research field. Here we describe a protocol to efficiently isolate mitochondria with high quality and purity from skeletal muscle of mice using Nycodenz density gradient ultracentrifugation.