%0 Journal Article
%T Isolation of Mitochondria from Murine Skeletal Muscle.
%A Dong L
%A Li X
%A Li A
%A Yi J
%A Zhou J
%J Methods Mol Biol
%V 2816
%N 0
%D 2024
%M 38977590
暂无%R 10.1007/978-1-0716-3902-3_8
%X Skeletal muscle is one of the largest tissues in human body. Besides enabling voluntary movements and maintaining body's metabolic homeostasis, skeletal muscle is also a target of many pathological conditions. Mitochondria occupy 10-15% volume of a muscle myofiber and regulate many cellular processes, which often determine the fate of the cell. Isolation of mitochondria from skeletal muscle provides opportunities for various multi-omics studies with a focus on mitochondria in biomedical research field. Here we describe a protocol to efficiently isolate mitochondria with high quality and purity from skeletal muscle of mice using Nycodenz density gradient ultracentrifugation.