Abstract：
In flowering plants, proper seed development is achieved through the constant interplay of fertilization products, embryo and endosperm, and maternal tissues. Understanding such a complex biological process requires microscopy techniques able to unveil the seed internal morphological structure. Seed thickness and relatively low permeability make conventional tissue staining techniques impractical unless combined with time-consuming dissecting methods. Here, we describe two techniques to imaging the three-dimensional structure of Arabidopsis seeds by confocal laser scanning microscopy. Both procedures, while differing in their time of execution and resolution, are based on cell wall staining of seed tissues with fluorescent dyes.
摘要：
在开花植物中，适当的种子发育是通过施肥产品的不断相互作用来实现的，胚胎和胚乳，和母体组织。了解这种复杂的生物过程需要能够揭示种子内部形态结构的显微镜技术。种子厚度和相对较低的渗透性使常规组织染色技术不切实际，除非与耗时的解剖方法结合使用。这里,我们描述了两种通过共聚焦激光扫描显微镜成像拟南芥种子的三维结构的技术。这两个程序，虽然他们的执行和解决时间不同，是基于用荧光染料对种子组织的细胞壁染色。
