Abstract：
Recent advancements in tissue engineering have witnessed luffa-derived scaffolds, exhibiting their exceptional potential in cellular proliferation, biocompatibility, appropriate interconnectivity, and biomechanical strength. In vivo studies involved implanting fabricated scaffolds subcutaneously in Wistar rats to evaluate their impact on the heart, liver, and kidneys. This approach provided a safe and minimally invasive means to evaluate scaffold compatibility with surrounding tissues. Male Wistar rats were categorized into four distinct groups, Group A, B, C, and D are referred to as 3% LC implanted scaffolds, 5% LC implanted scaffolds, control (without luffa scaffolds), and Sham (without any scaffold implantation), respectively. Histological analysis in all the groups indicated that the animal models did not exhibit any signs of inflammation or toxicity, suggesting favorable tissue response to the implanted scaffolds. Initial observations revealed elevated levels of enzymes and biomarkers in the experimental groups after a 24 h interval, including aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), bilirubin, creatine kinase-MB (CK-MB), and serum creatinine. However, these parameters normalized 3 weeks post-implantation, with no significant increase compared to the control groups, suggesting that the implanted luffa-based scaffolds did not induce adverse effects on the heart, liver, and kidneys. Furthermore, the scaffold\'s significant pore size and porosity enable it to release drugs, including antibacterial medications. This study demonstrates promising results, indicating excellent scaffold porosity, sustained drug release, affirming the in vivo biocompatibility, absence of inflammatory responses, and overall tissue compatibility highlighting the immense potential of these luffa-based scaffolds in various tissue engineering and regenerative medicine applications.
摘要：
组织工程的最新进展见证了丝瓜衍生的支架，在细胞增殖中表现出非凡的潜力，生物相容性，适当的互连，和生物力学强度。体内研究涉及在Wistar大鼠皮下植入人造支架，以评估其对心脏的影响，肝脏,还有肾脏.这种方法提供了一种安全且微创的方法来评估支架与周围组织的相容性。雄性Wistar大鼠分为四个不同的组，A组,B,C,和D被称为3%LC植入支架，5%LC植入支架，控制(没有丝瓜脚手架)，和假（没有任何支架植入），分别。所有组的组织学分析表明，动物模型没有表现出任何炎症或毒性的迹象，表明对植入的支架有良好的组织反应。最初的观察显示，在24小时间隔后，实验组中的酶和生物标志物水平升高，包括天冬氨酸氨基转移酶（AST），丙氨酸氨基转移酶（ALT），碱性磷酸酶(ALP),胆红素,肌酸激酶-MB（CK-MB），还有血清肌酐.然而,这些参数在植入后3周标准化，与对照组相比没有显着增加，表明植入的基于丝瓜的支架不会对心脏产生不良影响，肝脏,还有肾脏.此外，支架的显著孔径和孔隙率使其能够释放药物，包括抗菌药物。这项研究证明了有希望的结果，表明优异的支架孔隙率，持续药物释放，确认体内生物相容性，没有炎症反应，和整体组织相容性突出了这些基于丝瓜的支架在各种组织工程和再生医学应用中的巨大潜力。
