背景:丝瓜(丝瓜。)是葫芦科的重要经济作物,俗称丝瓜或蔬菜葫芦。它是一种主要在亚洲亚热带和热带地区发现的一年生异花授粉作物,澳大利亚,非洲,和美洲。丝瓜不仅作为一种蔬菜,而且还具有药用特性,包括消炎药,抗糖尿病药,和抗癌作用。此外,源自丝瓜的纤维在生物技术和建筑等各个领域得到了广泛的应用。然而,丝瓜枯萎病对其生产构成严重威胁,和现有的控制方法在成本效益和环境考虑方面被证明是无效的。因此,迫切需要开发抗枯萎病的丝瓜品种。单株GWAS(sp-GWAS)已被证明是快速有效鉴定与目标性状相关的数量性状基因座(QTL)的有前途的工具,以及紧密相连的分子标记。
结果:在这项研究中,来自73个丝瓜种的97个人,包括两个主要的丝瓜物种,对单株GWAS进行了调查丝瓜枯萎病抗性。利用双消化限制性位点相关DNA(ddRAD)方法,共鉴定出8,919个高质量的单核苷酸多态性(SNPs).分析揭示了两种丝瓜物种的抗枯萎病的潜力。从3个性状中鉴定出6个QTL,包括疾病进展曲线下的面积(AUDPC),假定的抗病QTL,是在丝瓜的第二条染色体上鉴定出来的.在连锁不平衡区域内,与LOC111009722同源的候选基因,编码过氧化物酶40,与黄瓜甜瓜的抗病性有关,已确定。此外,为了验证与来自sp-GWAS的抗性相关的标记的适用性,测试了另外一组21个单独的丝瓜植物,在检测丝瓜物种埃及伊玛利亚磨坊的易感方面具有93.75%的准确性。
结论:总之,这些发现暗示了基因组位置,这可能有助于丝瓜对镰刀菌的野生抗性,并可用于未来的丝瓜枯萎病抗性育种计划,旨在通过使用易感连锁的SNP标记开发抗枯萎病品种。
BACKGROUND: Luffa (
Luffa spp.) is an economically important crop of the Cucurbitaceae family, commonly known as sponge gourd or vegetable gourd. It is an annual cross-pollinated crop primarily found in the subtropical and tropical regions of Asia, Australia, Africa, and the Americas.
Luffa serves not only as a vegetable but also exhibits medicinal properties, including anti-inflammatory, antidiabetic, and anticancer effects. Moreover, the fiber derived from luffa finds extensive applications in various fields such as biotechnology and construction. However,
luffa Fusarium wilt poses a severe threat to its production, and existing control methods have proven ineffective in terms of cost-effectiveness and environmental considerations. Therefore, there is an urgent need to develop
luffa varieties resistant to Fusarium wilt. Single-plant GWAS (sp-GWAS) has been demonstrated as a promising tool for the rapid and efficient identification of quantitative trait loci (QTLs) associated with target traits, as well as closely linked molecular markers.
RESULTS: In this study, a collection of 97 individuals from 73 luffa accessions including two major
luffa species underwent single-plant GWAS to investigate
luffa Fusarium wilt resistance. Utilizing the double digest restriction site associated DNA (ddRAD) method, a total of 8,919 high-quality single nucleotide polymorphisms (SNPs) were identified. The analysis revealed the potential for Fusarium wilt resistance in accessions from both luffa species. There are 6 QTLs identified from 3 traits, including the area under the disease progress curve (AUDPC), a putative disease-resistant QTL, was identified on the second chromosome of luffa. Within the region of linkage disequilibrium, a candidate gene homologous to LOC111009722, which encodes peroxidase 40 and is associated with disease resistance in Cucumis melo, was identified. Furthermore, to validate the applicability of the marker associated with resistance from sp-GWAS, an additional set of 21 individual luffa plants were tested, exhibiting 93.75% accuracy in detecting susceptible of luffa species L. aegyptiaca Mill.
CONCLUSIONS: In summary, these findings give a hint of genome position that may contribute to luffa wild resistance to Fusarium and can be utilized in the future luffa wilt resistant breeding programs aimed at developing wilt-resistant varieties by using the susceptible-linked SNP marker.