PDF(Pubmed)
Abstract：
This study aimed to isolate and characterize a native strain of Beauveria bassiana, coded as Bv065, showcasing its potential as a biological control agent targeting the palm weevil Dynamis borassi. Originating from a naturally infected D. borassi specimen collected in southwestern Colombia, the fungus underwent molecular identification and was identified as B. bassiana, exhibiting high sequence similarity with known reference strains. The physiological characterization revealed that Bv065 thrived within a temperature range of 25 to 30 °C and a pH range of 6 to 9. Moreover, the key carbon sources that allow optimal growth of the strain were identified through metabolic profiling, including sucrose, D-mannose, and γ-amino-butyric acid. These findings offer strategic insights for scalability and formulation methodologies. Additionally, enzymatic analyses unveiled robust protease activity within Bv065, crucial for catalysing insect cuticle degradation and facilitating host penetration, thus accentuating its entomopathogenic potential. Subsequent evaluations exposed Bv065\'s pathogenicity against D. borassi, causing significant mortality within nine days of exposure, albeit exhibiting limited effectiveness against Rhynchophorus palmarum. This study underscores the importance of understanding optimal growth conditions and metabolic preferences of B. bassiana strains for developing effective biopesticides. The findings suggest Bv065 as a promising candidate for integrated pest management strategies in neotropical regions, particularly for controlling palm weevil infestations in coconut and peach palm cultivation. Future research avenues include refining mass production methodologies, formulating novel delivery systems, and conducting comprehensive field efficacy trials to unlock the full potential of Bv065 in fostering sustainable pest management practices. Overall, this study contributes to the growing body of knowledge on entomopathogenic fungi and their pivotal role in biological control, offering nuanced perspectives on eco-friendly alternatives to conventional insecticidal interventions.
摘要：
本研究旨在分离和鉴定球孢白僵菌的本地菌株，编码为Bv065，展示了其作为针对棕榈象鼻虫Dynamisborassi的生物防治剂的潜力。源自哥伦比亚西南部自然感染的D.borassi标本，该真菌经过分子鉴定，被鉴定为B.bassiana，与已知参考菌株表现出高序列相似性。生理表征显示Bv065在25至30°C的温度范围和6至9的pH范围内蓬勃发展。此外，通过代谢谱分析确定了允许菌株最佳生长的关键碳源，包括蔗糖，D-甘露糖,和γ-氨基丁酸。这些发现为可扩展性和制定方法提供了战略见解。此外,酶分析揭示了Bv065中强大的蛋白酶活性，对催化昆虫角质层降解和促进宿主渗透至关重要，从而增强了其昆虫致病的潜力。随后的评估暴露了Bv065对D.borassi的致病性，在暴露后九天内导致大量死亡，尽管对Rhynchophoruspalmarum的效果有限。这项研究强调了了解最佳生长条件和代谢偏好的重要性。研究结果表明，Bv065是新热带地区害虫综合管理策略的有希望的候选者，特别是用于控制椰子和桃树栽培中的棕榈象鼻虫侵染。未来的研究途径包括精炼大规模生产方法，制定新颖的输送系统，并进行全面的田间功效试验，以释放Bv065在促进可持续虫害管理实践中的全部潜力。总的来说,这项研究有助于对昆虫病原真菌及其在生物防治中的关键作用的知识不断增长，为传统杀虫干预措施的生态友好替代品提供细致入微的观点。
