褐藻入侵山东,中国,通过进口森林木材,对我国森林健康构成威胁。具有广泛环境耐受性的外来昆虫,包括低温,可能有更好的机会在冬天生存,成为入侵。了解H.lumiperda的耐寒策略可能有助于设计可持续的害虫管理方法。在这项研究中,我们的目的是调查越冬H.milniperda成虫的耐寒能力和相关生理指标,以确定任何可能的越冬策略。成虫H.lumiperda的过冷点(SCP)在数月内显着不同,并在越冬中期和越冬后达到最低水平,最低SCP-6.45±0.18°C。随着寒冷暴露温度的降低,成虫的存活率逐渐下降,没有成年人在-15°C下存活超过1天,1天的LLT50为-7.1°C。由于H.ligniperda成虫可以在内部冰形成中幸存下来,它们是耐冻昆虫。在整个越冬期间,SCP和水,蛋白质,山梨醇,成人甘油含量先下降后上升。我们报道了总蛋白质之间的显著相关性,山梨醇,海藻糖,甲虫和SCP中的甘油含量。糖原,脂质,蛋白质,海藻糖,成年甲虫的山梨醇含量可能直接影响其耐寒能力和冬季的存活。本研究为进一步研究木薯成虫的代谢和抗寒策略提供了生理生化基础。这可能有助于预测害虫的种群动态和分布潜力。
Hylurgus ligniperda invaded Shandong, China, through imported forest timber, posing a threat to China\'s forest health. Exotic insects with broad environmental tolerance, including low temperatures, may have a better chance of surviving the winters and becoming invasive. Understanding the cold-tolerance strategies of H. ligniperda may help to design sustainable pest management approaches. In this study, we aim to investigate the cold-tolerance ability and relevant physiological indicators in overwintering H. ligniperda adults to determine any possible overwintering strategies. Supercooling points (SCPs) for adults H. ligniperda differed significantly across months and reached the lowest level in the mid- and post-overwintering period, the minimum SCPs -6.45 ± 0.18 °C. As the cold exposure temperature decreased, the survival rate of adults gradually decreased, and no adult survived more than 1 day at -15 °C, and the LLT50 for 1 day was -7.1 °C. Since H. ligniperda adults can survive internal ice formation, they are freeze-tolerant insects. Throughout the overwintering period, the SCPs and the water, protein, sorbitol, and glycerol content in adults decreased initially and then increased. We reported significant correlations between total protein, sorbitol, trehalose, and glycerol content in the beetles and SCPs. Glycogen, lipid, protein, trehalose, and sorbitol content in adult beetles may directly affect their cold-tolerance capacity and survival during winter. This study provides a physiological and biochemical basis for further study of metabolism and cold-tolerance strategies in H. ligniperda adults, which may help predict population dynamics and distribution potential of pests.