Abstract：
BACKGROUND: Exercise or exercise capacity is a vital physiological function. It is known that certain cytokines support muscle function during exercise and, as a result, increase exercise capacity.
OBJECTIVE: In this study, the effect of metformin administered in combination with exercise on osteocalcin (OCN), insulin, and interleukin-6 (IL-6) levels in rats was investigated.
METHODS: Forty-two male Wistar rats were used in this study. The animals were randomly divided into six groups: control (CONT), only exercise (EXE), metformin_100 mg/kg (Met100), metformin_200 mg/kg (Met200), metformin_100 mg/kg+exercise (Met100+EXE), and metformin_200 mg/kg+exercise (Met200+EXE). A 10-week intervention was conducted, excluding exercise training. During the experiment, the groups receiving metformin application (100 or 200 mg/kg) were administered with metformin. At the end of the study, serum samples were collected from the rats to determine the levels of osteocalcin, insulin, and IL-6 using the enzyme-linked immunosorbent assay method. In addition, glucose levels and body weights were evaluated. GraphPad Prism was used for the analyses.
RESULTS: The OCN and insulin levels of the Met100+EXE and Met200+EXE groups were found to be higher compared to the CONT, Met100, and Met200 groups (P < 0.05). The IL-6 level of the EXE group was determined to be higher than that of the CONT, Met100, and Met200 groups (P < 0.01). It was observed that both exercise and the individual or combined application of metformin resulted in lower blood glucose levels compared to the CONT group. The mean body weight of the EXE group was higher than that of the other groups.
CONCLUSIONS: The combined application of metformin and exercise has increased osteocalcin and insulin levels compared to metformin application alone.
OBJECTIVE: In this study, the effect of metformin administered in combination with exercise on osteocalcin (OCN), insulin, and interleukin-6 (IL-6) levels in rats was investigated.
METHODS: Forty-two male Wistar rats were used in this study. The animals were randomly divided into six groups: control (CONT), only exercise (EXE), metformin_100 mg/kg (Met100), metformin_200 mg/kg (Met200), metformin_100 mg/kg+exercise (Met100+EXE), and metformin_200 mg/kg+exercise (Met200+EXE). A 10-week intervention was conducted, excluding exercise training. During the experiment, the groups receiving metformin application (100 or 200 mg/kg) were administered with metformin. At the end of the study, serum samples were collected from the rats to determine the levels of osteocalcin, insulin, and IL-6 using the enzyme-linked immunosorbent assay method. In addition, glucose levels and body weights were evaluated. GraphPad Prism was used for the analyses.
RESULTS: The OCN and insulin levels of the Met100+EXE and Met200+EXE groups were found to be higher compared to the CONT, Met100, and Met200 groups (P < 0.05). The IL-6 level of the EXE group was determined to be higher than that of the CONT, Met100, and Met200 groups (P < 0.01). It was observed that both exercise and the individual or combined application of metformin resulted in lower blood glucose levels compared to the CONT group. The mean body weight of the EXE group was higher than that of the other groups.
CONCLUSIONS: The combined application of metformin and exercise has increased osteocalcin and insulin levels compared to metformin application alone.
摘要：
背景：运动或运动能力是至关重要的生理功能。众所周知，某些细胞因子在运动过程中支持肌肉功能，因此,提高运动能力。
目的：在本研究中，二甲双胍联合运动对骨钙蛋白（OCN）的影响，胰岛素，和白细胞介素-6（IL-6）在大鼠中的水平进行了研究。
方法：本研究使用42只雄性Wistar大鼠。将动物随机分为6组:对照组(CONT),仅练习（EXE），二甲双胍_100mg/kg（Met100），二甲双胍_200mg/kg（Met200），二甲双胍_100mg/kg+运动（Met100+EXE），和二甲双胍_200mg/kg+运动(Met200+EXE)。进行了为期10周的干预，不包括运动训练。在实验过程中,接受二甲双胍应用(100或200mg/kg)的组给予二甲双胍.在研究结束时,从大鼠中收集血清样本以确定骨钙蛋白的水平，胰岛素，和IL-6使用酶联免疫吸附测定法。此外,评估血糖水平和体重.GraphPadPrism用于分析。
结果：发现Met100+EXE和Met200+EXE组的OCN和胰岛素水平高于CONT，Met100、Met200组(P<0.05)。EXE组IL-6水平高于CONT组,Met100、Met200组(P<0.01)。观察到,与CONT组相比,运动和单独或联合应用二甲双胍导致更低的血糖水平。EXE组的平均体重高于其他组。
结论：与单独应用二甲双胍相比，联合应用二甲双胍和运动可增加骨钙蛋白和胰岛素水平。
目的：在本研究中，二甲双胍联合运动对骨钙蛋白（OCN）的影响，胰岛素，和白细胞介素-6（IL-6）在大鼠中的水平进行了研究。
方法：本研究使用42只雄性Wistar大鼠。将动物随机分为6组:对照组(CONT),仅练习（EXE），二甲双胍_100mg/kg（Met100），二甲双胍_200mg/kg（Met200），二甲双胍_100mg/kg+运动（Met100+EXE），和二甲双胍_200mg/kg+运动(Met200+EXE)。进行了为期10周的干预，不包括运动训练。在实验过程中,接受二甲双胍应用(100或200mg/kg)的组给予二甲双胍.在研究结束时,从大鼠中收集血清样本以确定骨钙蛋白的水平，胰岛素，和IL-6使用酶联免疫吸附测定法。此外,评估血糖水平和体重.GraphPadPrism用于分析。
结果：发现Met100+EXE和Met200+EXE组的OCN和胰岛素水平高于CONT，Met100、Met200组(P<0.05)。EXE组IL-6水平高于CONT组,Met100、Met200组(P<0.01)。观察到,与CONT组相比,运动和单独或联合应用二甲双胍导致更低的血糖水平。EXE组的平均体重高于其他组。
结论：与单独应用二甲双胍相比，联合应用二甲双胍和运动可增加骨钙蛋白和胰岛素水平。
